Challenge to search for lung progenitor cells and to develop alveolar type II cells from human iPS cells

寻找肺祖细胞并从人类 iPS 细胞中培养肺泡 II 型细胞的挑战

• 批准号: 23591146
• 负责人: ITO Isao
• 金额: $ 3.41万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23591146/
• 关键词: iPS細胞; 肺胞上皮細胞; 組織幹細胞; サーファクタント; レポーター細胞; SPC; NKX2.1; ES細胞; SP-C; サーファクタントプロテインC; II型肺胞上皮細胞; 前腸細胞; FOXA2; SOX2; ヒトiPS細胞

In this study grant, we have established original methods to develop iPS cells into alveolar cells. The induced population expressed marker proteins such as surfactant protein-C, which are required for alveolar type II cells. In the process of developing induction methods, we have identified a surface marker for NKX2.1 positive cells. The marker enables us to isolate these cells and cells positive for this marker developed into a range of airway and alveolar epithelial cells. Our findings would contribute much for further research on inducing alveolar cell population from undiffereniated cells.

在这项研究资助中，我们建立了将 iPS 细胞培养成肺泡细胞的原创方法。诱导的群体表达标记蛋白，例如表面活性剂蛋白-C，这是 II 型肺泡细胞所需的。在开发诱导方法的过程中，我们鉴定了 NKX2.1 阳性细胞的表面标记。该标记使我们能够分离这些细胞，并且该标记呈阳性的细胞发育成一系列气道和肺泡上皮细胞。我们的研究结果将为进一步研究从未分化细胞诱导肺泡细胞群做出贡献。

项目成果

Association of eosinophilic inflammation with FKBP51 expression in sputum cells in asthma

哮喘痰细胞中嗜酸性粒细胞炎症与 FKBP51 表达的关系

• 发表时间: 2013
• 影响因子: 3.7
• 作者: Tajiri T; Matsumoto H; Niimi A; Ito I; Oguma T; Nakaji H; Inoue H; Iwata T; Nagasaki T; Kanemitsu Y; Petrova G; Mishima M
• 通讯作者: Mishima M

Generation of alveolar epithelial cells from human pluripotent stem cells

从人多能干细胞产生肺泡上皮细胞

• 发表时间: 2014
• 作者: Gotoh S; Ito I; et al
• 通讯作者: et al

ssociation of eosinophilic inflammation with FKBP51 expression in sputum cells in asthma.

哮喘痰细胞中嗜酸性粒细胞炎症与 FKBP51 表达的关联。

• DOI: 10.1371/journal.pone.0065284
• 发表时间: 2013
• 影响因子: 3.7
• 作者: Tajiri T;Matsumoto H;Niimi A;Ito I;Oguma T;Nakaji H;Inoue H;Iwata T;Nagasaki T;Kanemitsu Y;Petrova G;Mishima M
• 通讯作者: Mishima M

Clinical features and risk factors of tuberculosis in living-donor liver transplant recipients

活体肝移植受者结核病的临床特征及危险因素

• 发表时间: 2012
• 影响因子: 2.6
• 作者: Imai S; Ito Y; Hirai T; Imai H; Ito I; Maekawa K; Chin K; Ichiyama S; Uemoto S; Mishima M
• 通讯作者: Mishima M

Tazobactam/piperacillin for moderate-to-severe pneumonia in patients with risk for aspiration : comparison with imipenem/cilastatin

他佐巴坦/哌拉西林治疗有误吸风险的中重度肺炎患者：与亚胺培南/西司他丁的比较

• 发表时间: 2010
• 影响因子: 3.2
• 作者: Ito I; Kadowaki S; Tanabe N; Haruna A; Kase M; Yasutomo Y; Tsukino M; Nakai A; Matsumoto H; Niimi A; Chin K; Ichiyama S; Mishima M
• 通讯作者: Mishima M