Basic studies on development of a novel delivery system of materials into fish oocytes

鱼类卵母细胞新型材料输送系统开发的基础研究

• 批准号: 23580243
• 负责人: HIRAMATSU Naoshi
• 金额: $ 3.49万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23580243/
• 关键词: 魚類; 受容体標的輸送; ビテロジェニン; ビテロジェニン受容体; 蛋白質相互作用; 卵母細胞

A yolk precursor vitellogenin (Vg) is selectively incorporated from the maternal blood into oocyte following its binding to ovarian Vg receptor. This study demonstrated that Vg obtained from heterologous species, as well as Vg coupled with voluntary lipids, can be delivered to fish eggs and offsprings by injecting them into broodstocks. This indicated a potential use of fish Vg as a transporter of bioactive materials; the system can be universal to any fish species. This study also confirmed that such system was found to be applicable for the delivery of bioactive lipids and/or hydrophobic materials to eggs and offsprings across generations.

卵黄前体卵黄蛋白原 (Vg) 在与卵巢 Vg 受体结合后选择性地从母体血液掺入卵母细胞。这项研究表明，从异源物种获得的 Vg 以及与自愿脂质结合的 Vg 可以通过将其注射到亲鱼中而传递给鱼卵和后代。这表明鱼 Vg 有可能用作生物活性物质的转运蛋白；该系统适用于任何鱼类。这项研究还证实，这种系统适用于将生物活性脂质和/或疏水材料传递给卵子和后代。

项目成果

Ovarian expression and localization of a vitellogenin receptor with eight ligand binding repeats in the cutthroat trout (Oncorhynchus clarki)

具有八个配体结合重复序列的卵黄蛋白原受体在切喉鳟鱼（Oncorhynchus clarki）中的卵巢表达和定位

• DOI: 10.1016/j.cbpb.2013.07.005
• 发表时间: 2013
• 作者: Mizuta; H.; Luo; W.; Ito; Y.; Mushirobira; Y.; Todo; T.; Hara; A.; Reading; B. J.; Sullivan; C. V.; Hiramatsu; N.
• 通讯作者: N.

カットスロートトラウト Oncorhynchus clarki における2型ビテロジェニン転写産物および蛋白質の卵発達に伴う発現変化

克拉克鳟鱼卵发育过程中 2 型卵黄蛋白原转录物和蛋白质表达的变化

• 发表时间: 2013
• 作者: 筵平裕次;水田紘子;羅ブンシュ;盛田祐加;澤口小有美;松原孝博;平松尚志;東藤孝;原彰彦
• 通讯作者: 原彰彦

Ovarian yolk formation in fishes: Molecular mechanisms underlying formation of lipid droplets and vitellogenin-derived yolk proteins

鱼类卵巢卵黄形成：脂滴和卵黄蛋白原衍生卵黄蛋白形成的分子机制

• 发表时间: 2014
• 作者: Hiramatsu; N.; Todo; T.; Sullivan; C.V.; Reading; B.J; Matsubara; T.; Ryu; Y;Hara; A.
• 通讯作者: A.

Yolk formation in fish : Multiple vitellogenins and their receptors

鱼卵黄的形成：多种卵黄原蛋白及其受体

• 发表时间: 2013
• 作者: Hiramatsu N.; Mizuta H.; Luo W.; Nishimiya O.; Wu M.; Mushirobira Y.; Reading B.J.; Sullivan C.V.; Todo T.;Hara A.
• 通讯作者: Hara A.

Ligand binding properties of ovarian lipoprotein receptors in the cutthroat trout

鳟鱼卵巢脂蛋白受体的配体结合特性

• 发表时间: 2014
• 作者: Mushirobira. Y.; Mizuta; H.; Luo; W.; Todo; T.; Hara; A.; Reading; B.J.; Sullivan; C.V.;Hiramatsu; N.
• 通讯作者: N.