Interactions of hydrophobic and hydrophilic semiconductor quantum dots with cell model systems for liver and adipose tissue (NANOFATE)

疏水性和亲水性半导体量子点与肝脏和脂肪组织细胞模型系统的相互作用（NANOFATE）

• 批准号: 57689413
• 负责人: Professor Dr. Alexander Eychmüller
• 金额: --
• 依托单位: Professur für Physikalische Chemie
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2008
• 资助国家: 德国
• 起止时间: 2007-12-31 至 2013-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/57689413?language=en
• 关键词: Interactions; hydrophobic; hydrophilic; semiconductor; quantum

Semiconductor quantum dots (QDs) and superparamagnetic iron-oxide nanocrystals (SPIOs) have exceptional physical properties that are well-suited for biomedical applications in vitro and in vivo. The direct injection of nanocrystals for imaging and therapy represents an important entry route into humans in future. Therefore, it is crucial to carefully investigate the biological response of the body to nanocrystals to avoid harmful side effects. In recent years we established a system to prepare nanocrystals with hydrophilic or hydrophobic properties which survive biological conditions in vitro and in vivo. The impact of these particles on intracellular processes in cell model systems for liver and adipose tissues have been investigated within the first funding period but it still remains unclear whether these findings can be translated to the situation in vivo.The goal of the current application is to investigate in vivo the interaction, processing and degradation of nanocrystals as well as the specific cellular response to these interactions after injection of nanocrystals in mice. To address these issues we will• determine the contributions of different hepatic cell types for nanocrystals clearance.• Follow the intracellular routes of nanocrystals within hepatic cells.• quantify the degradation and excretion of nanocrystals.• explore biological responses to nanocrystals in relation to cell type and intracellular localization in the liver.The outcome of these results will not only provide in depth insights into potential hazards associated with unintended exposure of nanocrystals but will also greatly influence the potential use of nanocrystals for biomedical applications.

半导体量子点（QD）和超帕磁铁氧化纳米晶体（SPIO）具有出色的物理特性，非常适合在体外和体内生物医学应用。直接注射用于成像和治疗的纳米晶体代表了将来进入人类的重要进入途径。因此，仔细研究人体对纳米晶体的生物学反应至关重要，以避免有害副作用。近年来，我们建立了一个系统，以制备具有亲水性或疏水性能的纳米晶体，该纳米晶体在体外和体内生存生物学条件。这些颗粒对细胞模型系统中肝和脂肪时序的细胞内过程的影响已在第一筹期内进行了研究，但仍然不清楚这些发现是否可以转化为体内的情况。当前应用的目的是在体内调查nanocrystals的相互作用，处理和去分别的互动互动的相互作用，处理和去分布的互动，以及这些特定的互动。 To address these issues we will• determine the contributions of different hepatitic cell types for nanocrystals clearance.• Follow the intracellular routes of nanocrystals within hepatitic cells.• Quantify the degradation and excretion of nanocrystals.• Explore biological responses to nanocrystals in relation to cell type and intracellular localization in the liver.The outcome of these results will not only provide depth对与纳米晶体的意外暴露有关的潜在危害的见解，但也会极大地影响纳米晶体在生物医学应用中的潜在使用。