Interactions of hydrophobic and hydrophilic semiconductor quantum dots with cell model systems for liver and adipose tissue (NANOFATE)

疏水性和亲水性半导体量子点与肝脏和脂肪组织细胞模型系统的相互作用（NANOFATE）

• 批准号: 57689413
• 负责人: Professor Dr. Alexander Eychmüller
• 金额: --
• 依托单位: Professur für Physikalische Chemie
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2008
• 资助国家: 德国
• 起止时间: 2007-12-31 至 2013-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/57689413?language=en
• 关键词: Interactions; hydrophobic; hydrophilic; semiconductor; quantum

Semiconductor quantum dots (QDs) and superparamagnetic iron-oxide nanocrystals (SPIOs) have exceptional physical properties that are well-suited for biomedical applications in vitro and in vivo. The direct injection of nanocrystals for imaging and therapy represents an important entry route into humans in future. Therefore, it is crucial to carefully investigate the biological response of the body to nanocrystals to avoid harmful side effects. In recent years we established a system to prepare nanocrystals with hydrophilic or hydrophobic properties which survive biological conditions in vitro and in vivo. The impact of these particles on intracellular processes in cell model systems for liver and adipose tissues have been investigated within the first funding period but it still remains unclear whether these findings can be translated to the situation in vivo.The goal of the current application is to investigate in vivo the interaction, processing and degradation of nanocrystals as well as the specific cellular response to these interactions after injection of nanocrystals in mice. To address these issues we will• determine the contributions of different hepatic cell types for nanocrystals clearance.• Follow the intracellular routes of nanocrystals within hepatic cells.• quantify the degradation and excretion of nanocrystals.• explore biological responses to nanocrystals in relation to cell type and intracellular localization in the liver.The outcome of these results will not only provide in depth insights into potential hazards associated with unintended exposure of nanocrystals but will also greatly influence the potential use of nanocrystals for biomedical applications.

半导体量子点（QD）和超顺磁性氧化铁纳米晶体（SPIO）具有卓越的物理特性，非常适合体外和体内生物医学应用，直接注射纳米晶体进行成像和治疗是进入人体的重要途径。因此，仔细研究身体对纳米晶体的生物反应以避免有害的副作用至关重要。近年来，我们建立了一个准备系统。具有亲水或疏水特性的纳米晶体可以在体外和体内的生物条件下生存。这些颗粒对肝脏和脂肪组织细胞模型系统中细胞内过程的影响已在第一个资助期内进行了研究，但仍不清楚这些发现是否可以。当前应用的目标是研究体内纳米晶体的相互作用、加工和降解，以及在小鼠体内注射纳米晶体后对这些相互作用的特定细胞反应。我们将解决的问题• 确定不同肝细胞类型对纳米晶体清除的贡献。• 追踪肝细胞内纳米晶体的细胞内路径。• 量化纳米晶体的降解和排泄。• 探索纳米晶体与细胞类型和细胞内定位相关的生物反应这些结果不仅将深入了解与纳米晶体意外暴露相关的潜在危害，而且还将极大地影响纳米晶体在生物医学应用中的潜在用途。