黄色猩々蝿のＴＡＬＥモジュレーションを利用した転写因子群の二機能性に関する研究

黄斑果蝇TALE调控转录因子双功能研究

• 批准号: 18F18792
• 负责人: ラスカム ニコラス
• 金额: $ 1.34万
• 依托单位: Okinawa Institute of Science and Technology Graduate University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2018
• 资助国家: 日本
• 起止时间: 2018-10-12 至 2020-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18F18792/
• 关键词: GENE REGULATION; STATISTICAL MODELLING; SYSTEMS BIOLOGY; DROSOPHILA MELANOGASTER

In FY2019, the fellow’s role was to develop the experimental model to test synthetic regulatory circuits using transcription activator-like (TALE) proteins fused to activator or repressor domains (Crocker et al., 2016). The amount of the TFs contribution is manipulated by adding or subtracting the number of TALE-binding sites in the enhancer. The same approach with TALE fused to Bcd was applied; by adjusting the number of TALE binding sites, the impact of the stepwise increase in Bcd activity will be observed, so testing whether and at what point it switches from repressor to activator. Experimental conditions for generating and assembling essential fragments that compose of transgenic constructs were optimized. They can then be used to create transgenic flies for applications later on. Altogether, the success of these steps provides a strong technical basis and shows great promises for downstream experiments.

在 2019 财年，该研究员的职责是开发实验模型，以使用与激活子或阻遏子结构域融合的转录激活子样 (TALE) 蛋白来测试合成调节电路（Crocker 等人，2016）。添加或减少增强子中 TALE 结合位点的数量 应用与 Bcd 融合的 TALE 相同的方法； TALE 结合位点，将观察到 Bcd 活性逐步增加的影响，因此可以测试它是否以及在什么点从阻遏物转换为激活物，然后可以优化用于生成和组装组成转基因构建体的必需片段的实验条件。总而言之，这些步骤的成功为后续的应用提供了坚实的技术基础，并显示出巨大的前景。