黄色猩々蝿のＴＡＬＥモジュレーションを利用した転写因子群の二機能性に関する研究

黄斑果蝇TALE调控转录因子双功能研究

• 批准号: 18F18792
• 负责人: ラスカム ニコラス
• 金额: $ 1.34万
• 依托单位: Okinawa Institute of Science and Technology Graduate University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2018
• 资助国家: 日本
• 起止时间: 2018-10-12 至 2020-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18F18792/
• 关键词: GENE REGULATION; STATISTICAL MODELLING; SYSTEMS BIOLOGY; DROSOPHILA MELANOGASTER

In FY2019, the fellow’s role was to develop the experimental model to test synthetic regulatory circuits using transcription activator-like (TALE) proteins fused to activator or repressor domains (Crocker et al., 2016). The amount of the TFs contribution is manipulated by adding or subtracting the number of TALE-binding sites in the enhancer. The same approach with TALE fused to Bcd was applied; by adjusting the number of TALE binding sites, the impact of the stepwise increase in Bcd activity will be observed, so testing whether and at what point it switches from repressor to activator. Experimental conditions for generating and assembling essential fragments that compose of transgenic constructs were optimized. They can then be used to create transgenic flies for applications later on. Altogether, the success of these steps provides a strong technical basis and shows great promises for downstream experiments.

在2019财年，该同伴的作用是开发实验模型，使用融合到激活剂或复制剂结构域的转录激活剂（Tale）蛋白（Crocker等，2016）来测试合成调节回路。通过添加或减去增强子中的故事结合位点的数量来操纵TFS贡献的数量。与BCD融合的故事相同的方法也被应用了；通过调整故事结合位点的数量，将观察到BCD活性的逐步增加的影响，因此测试它是否以及在什么时候从阻遏物转换为激活剂。优化了用于产生和组装组成转基因构建体的必需片段的实验条件。然后，它们可用于以后为应用创建转基因苍蝇。总的来说，这些步骤的成功提供了强大的技术基础，并为下游实验带来了巨大的承诺。