Cartilage repair using a scaffold free tissue engineered construct derived from multipotent stem cell

使用源自多能干细胞的无支架组织工程构建体进行软骨修复

• 批准号: 22390291
• 负责人: NAKAMURA Norimasa
• 金额: $ 11.65万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22390291/
• 关键词: ES cell; iPS cell; stem cell; 軟骨再生; Scaffold; 軟骨分化; 骨分化; Stem cell; scaffold; 幹細胞; 組織工学; 骨再生; スキャフォールド

We had developed a scaffold-free tissue-engineered construct (TEC) derived from synovial mesenchymal stem cells(Sy-MSC,Sy-TEC). Sy-TEC promoted cartilage repair in a porcine injury model. However, repaired cartilage by Sy-TEC has limited morphology in animal model especially in superficial zone.Embryonic stem cell(ESC) is expected as a better cell source for cartilage repair. We have made TEC derived from ESC (ES-TEC) and performed chondrogenic differentiation in vitro. Expression level of chondrogenic genes were significantly higher compared to Sy-TEC and equal to native chondrocyte.We have transplanted TEC/B-TCP complex to osteochondral defect of rabbit femoral groove, and then performed histological assessment. Repair tissue by Sy-TEC was like fibrocartilage but that by ES-TEC was hyaline cartilage like morphology at early stage following surgery. These results suggest the feasibility of ES-TEC in cartilage repair.We also succeeded in developing the TEC derived from iPS cells.

我们开发了一种源自滑膜间充质干细胞的无支架组织工程构建体（TEC）（Sy-MSC，Sy-TEC）。 Sy-TEC 促进猪损伤模型中的软骨修复。然而，Sy-TEC修复的软骨在动物模型中形态有限，尤其是浅层区域。胚胎干细胞（ESC）有望成为更好的软骨修复细胞来源。我们制备了ESC衍生的TEC（ES-TEC），并在体外进行了软骨分化。成软骨基因的表达水平明显高于Sy-TEC，与天然软骨细胞相当。我们将TEC/B-TCP复合物移植到兔股骨沟骨软骨缺损处，然后进行组织学评估。 Sy-TEC 的修复组织类似于纤维软骨，而 ES-TEC 的修复组织在术后早期的形态是透明软骨样的。这些结果表明ES-TEC在软骨修复中的可行性。我们还成功开发了源自​​iPS细胞的TEC。

项目成果

Treatment of Partial Growth Arrest Using an In Vitro-generated Scaffold-free Tissue-engineered Construct Derived From Rabbit Synovial Mesenchymal Stem Cells

使用源自兔滑膜间充质干细胞的体外生成的无支架组织工程构建体治疗部分生长停滞

• 发表时间: 2012
• 作者: Yoshida K; Higuchi C; Nakamura N; et. Al
• 通讯作者: et. Al

Development of scaffold-free tissue-engineered construct (TEC) with chondrogenic differentiation capacity using rabbit embryonic stem cell-derived mesenchymal stem cells

使用兔胚胎干细胞衍生的间充质干细胞开发具有软骨分化能力的无支架组织工程构建体（TEC）

• 发表时间: 2012
• 作者: Moriguchi Y; Shimomura K; Teramura T; Ando W; Sakaue M; Hasegawa H; Sugita N; Shino K; Yoshikawa H; Nakamura N
• 通讯作者: Nakamura N

Scaffold-free Tissue Engineered Construct(TEC)derived from synovial mesenchymal stem cells in musculoskeletal regeneration

来自滑膜间充质干细胞的无支架组织工程构建体（TEC）在肌肉骨骼再生中的应用

• 发表时间: 2010
• 作者: 中村憲正

ウサギ胚性幹細胞より誘導された間葉系幹細胞由来スキャフォールド三次元人工組織による軟骨再生

使用来源于兔胚胎干细胞的间充质干细胞支架三维人工组织进行软骨再生

• 作者: 森口悠;中村憲正
• 通讯作者: 中村憲正

ヒト滑膜由来間葉系幹細胞から作成した三次元人工組織(TEC)の移植前保存条件の検討

由人滑膜来源的间充质干细胞创建的三维工程组织 (TEC) 移植前的储存条件检查

• 作者: 阪上守人;中村憲正
• 通讯作者: 中村憲正