Analysis of the origin and properties of dental and bone marrow mesenchymal stem cells by using mice to label NC-derived and mesodermal cells

使用小鼠标记 NC 衍生细胞和中胚层细胞分析牙齿和骨髓间充质干细胞的起源和特性

• 批准号: 21659422
• 负责人: YAMAZAKI Hidetoshi
• 金额: $ 2.07万
• 依托单位: Mie University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21659422/
• 关键词: 神経堤細胞; 中胚葉; 間葉系細胞; 歯髄細胞; 骨髄間葉系細胞; 象牙芽細胞; 胚性幹細胞; 細胞系譜追跡; 間葉細胞; 歯髄; 骨髄; 間葉系幹細胞; 造血支持能; CFU-F; 骨髄細胞; 幹細胞; 歯; 分化能

By using transgenic mouse which enabled us to trace NC-derived or mesodermal cells, we showed that dental mesenchyme was composed of mainly NC-derived cells, whereas half of bone marrow(BM) mesenchyme was composed of cells other than NC-derived and mesodermal cells. Furthremore, a colony-forming unit-fibroblast(CFU-F) assay indicated that CFU-Fs in the dental pulp are derived from NC, and BM are derived from both NC and mesodermal cells. Furthermore, we showed that dental mesenchyme supported the differentiation of B lymphocytes and osteoclasts.

通过使用使我们能够追踪 NC 来源或中胚层细胞的转基因小鼠，我们发现牙齿间质主要由 NC 来源的细胞组成，而一半的骨髓 (BM) 间质由除 NC 来源和中胚层以外的细胞组成细胞。此外，集落形成单位成纤维细胞（CFU-F）测定表明牙髓中的CFU-F源自NC，而BM源自NC和中胚层细胞。此外，我们发现牙齿间充质支持 B 淋巴细胞和破骨细胞的分化。

项目成果

Characterization of the earliest hematolymphoid progenitors in mouse ontogeny

小鼠个体发育中最早的造血淋巴祖细胞的特征

• 发表时间: 2011
• 作者: 山根利之;鷲野亜矢;山崎英俊
• 通讯作者: 山崎英俊

Involvement of p38a in blood cells in the regulation o obesity and blood glucose level

血细胞中 p38a 参与肥胖和血糖水平的调节

• 发表时间: 2010
• 作者: Sadatugu Ookuma; Takahiro Fujikawa; Kazuto Sugimura; Toshiyuki Yamane; Hidetoshi Yamazaki; Kinya Otsu; Masato Ogata
• 通讯作者: Masato Ogata

Developmental origin of the earliest mouse hematolympoid progenitors

最早的小鼠血淋巴祖细胞的发育起源

• 发表时间: 2010
• 作者: Yamane Toshiyuki; Yamazaki Hidetoshi
• 通讯作者: Yamazaki Hidetoshi

神経堤細胞と器官形成

神经嵴细胞和器官形成

• 发表时间: 2009
• 作者: 山崎英俊

Developmental origin of the earliest definitive hematopoietic progenitors

最早的定型造血祖细胞的发育起源

• 发表时间: 2009
• 作者: Yamane Toshiyuki; Yamazaki Hidetoshi
• 通讯作者: Yamazaki Hidetoshi