Apicomplexa specific traffic pathway in protozoa

原生动物中顶端复合体特定的交通途径

• 批准号: 21790411
• 负责人: NAKANO Yumiko
• 金额: $ 2.75万
• 依托单位: National Institute of Infectious Diseases
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21790411/
• 关键词: 原虫; small GTPase; メンブレントラフィック; Rab5; アピコンプレキサ; Plasmodium falciparu; 感染症; Plasmodium falciparum

One of the small GTPase, PfRab5a, was previously reported that involvement of endocytosis of erythrocyte cytosol. This work shows that another Rab5 isotype, Rab5b, possesses conserved motif with myristoilation site in N-terminus. Rab5b homologue is presented in Plasmodium and Toxopasma but not in Cryptosporidium, Paramecium and Tetrahymena in protozoa classified in Alveolata. Although expression of PfRab5b was low among three Rab5 isotypes, PfRab5b was expressed in erythrocytic stage. Expression of recombinant protein of PfRab5b was effective when an artificial gene appropriate for E. coli K12 codon was used.

先前报道小GTP酶之一PfRab5a参与红细胞胞质的内吞作用。这项工作表明，另一种 Rab5 同种型 Rab5b 具有保守基序，N 末端有肉豆蔻油化位点。 Rab5b 同源物存在于疟原虫和弓形虫中，但不存在于分类为肺泡动物门的原生动物中的隐孢子虫、草履虫和四膜虫中。尽管PfRab5b的表达在三种Rab5同种型中较低，但PfRab5b在红细胞阶段表达。当使用适合大肠杆菌K12密码子的人工基因时，PfRab5b重组蛋白的表达是有效的。

项目成果

Marked Amplification and Diversification of Products of ras Genes from Rat Brain, Rab GTPases, in the Ciliates Tetrahymena thermophila and Paramecium tetraurelia.

在纤毛虫嗜热四膜虫和草履虫中，大鼠脑 ras 基因、Rab GTPases 的产物显着扩增和多样化。

• 发表时间: 2010
• 作者: Saito

Marked Amplification and Diversification of Products of ras Genes from Rat Brain, Rab GTPases, in the Ciliates Tetrahymena thermophila and Paramecium tetraurelia

纤毛虫嗜热四膜虫和草履虫中大鼠脑 ras 基因、Rab GTPases 的显着扩增和产物多样化

• DOI: 10.1111/j.1550-7408.2010.00503.x
• 发表时间: 2010-09-01
• 期刊: Journal of Eukaryotic Microbiology
• 影响因子: 2.2
• 作者: Y. Saito;Tohru Nakahara;K. Nakano;T. Nozaki;O. Numata
• 通讯作者: O. Numata

Diversity and Significance of Vesicular Trafficking in Entamoeba histolytica.

溶组织内阿米巴囊泡贩运的多样性和意义。

• 发表时间: 2010
• 作者: Yumiko Saito