A fundamental study regarding the cultured periosteum and its scaffold leading to a novel periodontal regenerative treatment

关于培养骨膜及其支架的基础研究导致新型牙周再生治疗

• 批准号: 21390554
• 负责人: OKUDA Kazuhiro
• 金额: $ 11.9万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21390554/
• 关键词: 歯周組織; 再生医学; 細胞治療; 再生骨膜; 細胞培養; 生体材料; 基材; 硬組織評価; 培養骨膜シート; 多孔性ポリ乳酸膜; 接着分子; 冷凍保存; アルカリホスファターゼ; カテキン; 抗酸化作用; 近赤外線イメージング; 骨膜細胞; ハイドロキシアパタイト; 石灰化; ポリ乳酸カプロラクトン重合体; cambium layer; 幹細胞

A novel periodontal regenerative treatment using autologous cultured periosteal sheet with an osteogenic property attracts attention nowadays.We examined the cell-biological characteristics of this sheet and the reaction of various scaffolding materials to this biological sheet through in vitro and in vivo experiments. It was suggested that cultured periosteal sheet has stem cell-like cells, and has a possibility to express osteogenic property by induction of cell differentiation. Cell adhesion was improved by making processing some treatment on the surface of high molecular or inorganic scaffolding materials, and was able to support a cell migration and calcification.

使用具有成骨特性的自体培养骨膜片的新型牙周再生治疗如今引起了人们的关注。我们通过体外和体内实验研究了该片的细胞生物学特性以及各种支架材料对该生物片的反应。有人提出，培养的骨膜片具有干细胞样细胞，并且有可能通过诱导细胞分化来表达成骨特性。通过对高分子或无机支架材料的表面进行一些处理，可以提高细胞的粘附性，并能够支持细胞的迁移和钙化。

项目成果

A clinical study of alveolar bone tissue engineering with cultured autogenous periosteal cells : coordinated activation of bone formation and resorption

培养自体骨膜细胞牙槽骨组织工程的临床研究：骨形成和骨吸收的协调激活

• DOI: 10.1016/j.bone.2012.02.631
• 发表时间: 2012
• 影响因子: 4.1
• 作者: Masaki Nagata

Collagen-coated poly(L-lactide-co-ε-caprolactone) film : A promising scaffold for cultured periosteal sheets

胶原蛋白涂层聚（L-丙交酯-共-ε-己内酯）薄膜：一种有前景的培养骨膜片支架

• 发表时间: 2010
• 作者: Kawase T; Yamanaka K; Suda Y; Kaneko T; Okuda K; Kogami H; Nakayama H; Nagata M; (他2名;1; 5; 6; 8番目)
• 通讯作者: 8番目)

A short-term preservation of human cultured periosteal sheets, osteogenic grafting materials, using a commercial preservation sulotion containing epigallocatechin-3-gallate(Theliokeep) under chilled sonditions

使用含有表没食子儿茶素-3-没食子酸酯（Theliokeep）的商业保存液在冷冻条件下短期保存人类培养的骨膜片、成骨移植材料

• 影响因子: 1.6
• 作者: Kamiya M; Kawase T; Kobayashi M; Sekine Yu; Okuda K; Nagata M(他4名;2; 5; 6番目)
• 通讯作者: 6番目)

Evaluation by bone scintigraphy of osteogenic activity of commercial bioceramics(porous β-TCP and HAp particles)subcutaneously implanted in rats

骨显像评价大鼠皮下植入商业生物陶瓷（多孔β-TCP和HAp颗粒）的成骨活性

• 作者: Hitoshi Nakayama

治療用ヒト培養骨膜シートの凍結保存法

治疗性人培养骨膜片的冷冻保存方法

• 发表时间: 2011
• 作者: 川瀬知之