Study on novel tissue regenerative therapy using bone marrow-derived mesenchymal stem cells and FGF/Phosphophoryn/Collagen complex

利用骨髓间充质干细胞和 FGF/磷酸化/胶原复合物进行新型组织再生疗法的研究

• 批准号: 21592630
• 负责人: FUJII Takeo
• 金额: $ 2.91万
• 依托单位: Matsumoto Dental University (2011)Health Sciences University of Hokkaido (2009-2010)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21592630/
• 关键词: 歯周外科学; 再生治療; 三次元培養系; 歯髄間葉系幹細胞; ホスホホリン; FGF; 再生医療; 歯周組織再生; 骨髄間葉系幹細胞

With the goal to establish new tissue regenerative therapy for extensive regeneration of destroyed periodontal tissues accompanying vertical or horizontal bone defects due to severe periodontitis, The aim of this study was to evaluate the kinetics of bone marrow-derived mesenchymal stem cells cultured in a FGF. collagen complex as a regenerative scaffold in vitro. Bone marrow-derived mesenchymal stem cells showed good growth in the collagen gel culture. The effect of FGF in promoting cell growth is exhibited at the early stage of culture, and the ALP activity has already passed the peak when assayed at 14 days of culture at FGF concentrations of 10 ng/ml. The ALP activity showed in the presence of phosphophoryn in a dose-dependent manner at 14 days of culture. A marked difference in calcium content was not observed. The present study sheds light on the development of an optimal biomaterial that forms the basis of calcified hard tissue formation, suggesting the feasibility of applying the 3-D collagen gel cell culture system for the construction of a scaffold that controls the amount of regenerating tissue.

为了建立新的组织再生疗法，用于严重牙周炎导致的伴有垂直或水平骨缺损的破坏牙周组织的广泛再生，本研究的目的是评估在 FGF 中培养的骨髓间充质干细胞的动力学。胶原复合物作为体外再生支架。骨髓间充质干细胞在胶原凝胶培养物中显示出良好的生长。 FGF促进细胞生长的作用在培养初期就显现出来，在FGF浓度为10 ng/ml时，培养14天时ALP活性已过峰值。培养 14 天时，在存在磷酸化蛋白的情况下，ALP 活性呈剂量依赖性。没有观察到钙含量的显着差异。本研究揭示了形成钙化硬组织形成基础的最佳生物材料的开发，表明应用 3-D 胶原凝胶细胞培养系统构建控制再生组织数量的支架的可行性。

项目成果

Periodontal Tissue Regeneration by the Application of FGF-2 in Periodontitis Patients

FGF-2在牙周炎患者中应用的牙周组织再生

• 发表时间: 2010
• 作者: T.Fujii; Y.Terada; M.Akamatsu; M.Watanuki
• 通讯作者: M.Watanuki

FGF-2 stimulates periodontal regeneration : results of a multi-center randomized clinical trial

FGF-2刺激牙周再生：多中心随机临床试验结果

• DOI: 10.1177/0022034510384616
• 发表时间: 2011
• 影响因子: 7.6
• 作者: Kitamura M; Akamatsu M; Machigashira M; 他35名
• 通讯作者: 他35名

Periodontal Tissue Regeneration by the Application of FGF-2 in Periodontitis Patients.

FGF-2 在牙周炎患者中的应用实现牙周组织再生。

• 发表时间: 2009
• 作者: T.Fujii; Y.Terada; M.Akamatsu; M.Watanuki.
• 通讯作者: M.Watanuki.

Periodontal Tissue Regeneration by the Application of FGF-2 in Patients

FGF-2在患者中应用的牙周组织再生

• 发表时间: 2010
• 作者: T. Fujii; Y. Terada; M. Akamatsu; M. Watanuki
• 通讯作者: M. Watanuki

歯周炎罹患歯に対するFGF-2投与の長期的効果および安全性の検討

检查 FGF-2 给药对受牙周炎影响的牙齿的长期影响和安全性

• 发表时间: 2012
• 作者: 北村正博;古市保志;藤井健男;川浪雅光;国松和司;島内英俊;山田了;小方頼昌;和泉雄一;伊藤公一;中川種昭;新井高;山崎和久;吉江弘正;野口俊英;渋谷俊昭;高柴正悟;栗原英見;永田俊彦;横田誠;前田勝正;廣藤卓雄;坂上竜資;原宣興;野口和行;小笠原健文;村上伸也
• 通讯作者: 村上伸也