Structural Chemistry on Information Transduction through Allosteric Effects in Heme Proteins

通过血红素蛋白变构效应进行信息转导的结构化学

• 批准号: 21350098
• 负责人: KITAGAWA Teizo
• 金额: $ 12.15万
• 依托单位: University of Hyogo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21350098/
• 关键词: 生物無機化学; 振動分光学; アロステリック効果; ガスセンサー蛋白; ヘモグロビン; 情報伝達; 生体分子化学; 生物物理; 共鳴ラマン; ヘム蛋白質; 紫外共鳴ラマン; 生体生命情報学; 生体分子科学; 紫外共鳴ラマン散乱

Structural chemistry involved in allosteric effects of heme proteins was investigated with visible and ultraviolet resonance Raman spectroscopy. About hemoglobin as a fundamental molecule, a quaternary structure of half-ligated state was quantitatively determined on the basis of inter-subunit interactions. As applications, information transduction pathway and its dynamics were elucidated with two oxygen sensor proteins, DOS and HemAT, and structural effects of effector binding was revelaed for an NO sensor protein, sGC. For respiration enzyme, proton pumping mechanism of a mammalian cytochrome c oxidase was examined.

利用可见光和紫外共振拉曼光谱研究了血红素蛋白变构效应中涉及的结构化学。对于血红蛋白作为基本分子，基于亚基间相互作用定量确定了半连接状态的四级结构。作为应用，利用两种氧传感器蛋白 DOS 和 HemAT 阐明了信息转导途径及其动力学，并揭示了 NO 传感器蛋白 sGC 的效应器结合的结构效应。对于呼吸酶，检查了哺乳动物细胞色素c氧化酶的质子泵送机制。

项目成果

A new way for understanding quaternary structure change of hemoglobin upon ligand binding on the basis of UV-resonance Raman evaluation of inter-subunit interactions

基于亚基间相互作用的紫外共振拉曼评估，了解配体结合后血红蛋白四级结构变化的新方法

• 发表时间: 2011
• 影响因子: 15
• 作者: Shigenori Nagatomo; et al.
• 通讯作者: et al.

Differences between two active forms of CO-bound soluble guanylate cyclase in the presence of activators and substrate and their populations revealed by resonance Raman spectroscopy

共振拉曼光谱揭示了存在激活剂和底物的两种活性形式的CO结合可溶性鸟苷酸环化酶及其群体之间的差异

• 发表时间: 2011
• 作者: B.Pal; T.Kitagawa
• 通讯作者: T.Kitagawa

Resonance Raman study on the oxygenated and the ferryl-oxo species of indoleamine 2, 3-dioxygenase during catalytic turnover

催化周转过程中吲哚胺 2, 3-双加氧酶的含氧和含氧物种的共振拉曼研究

• 发表时间: 2011
• 作者: Sachiko Yanagisawa; et al.
• 通讯作者: et al.

The β-sheet structure pH dependence of the core fragments of β_2-microglobulin amyloid fibrils

β_2-微球蛋白淀粉样原纤维核心片段的β-折叠结构pH依赖性

• 发表时间: 2010
• 作者: M.Hiramatsu; M.Lu; Y.Goto; T.Kitagawa
• 通讯作者: T.Kitagawa

Important Roles of Tyr43 at the Putative Heme Distal Side in Oxygen Recognition and Stability of the Fe(II)-O_2 Complex of YddV, a Globin-coupled Heme-based Oxygen Sensor Diguanylate Cyclase

推定血红素远端的 Tyr43 在 YddV（球蛋白偶联血红素氧传感器二鸟苷酸环化酶）的 Fe(II)-O_2 复合物的氧识别和稳定性中的重要作用

• 发表时间: 2010
• 影响因子: 2.9
• 作者: Kenichi Kitanishi; et al.
• 通讯作者: et al.