臨界ゲルクラスターを用いた巨大生体分子分離媒体の創製

使用关键凝胶簇创建巨型生物分子分离介质

• 批准号: 16J09442
• 负责人: Khairulina Kateryna (2017)
• 金额: $ 0.83万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2016
• 资助国家: 日本
• 起止时间: 2016-04-22 至 2018-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16J09442/
• 关键词: polymer gel; polyelectrolytes; dynamics; electrophoresis

Mobility of charged solutes in polymer networks is governed by diffusion and electrically driven migration, the latter one excessively utilized in gel electrophoresis. However, the physical mechanism of the migration is still not clear yet due to the uncontrollability of the number of structural parameters influencing migration, resulting in no clear understanding of solute-matrix interactions. In this thesis we used tetra-PEG gel as a model system and investigated the influence of structural parameters on migration of DNA molecules under constant electric field conditions. The effects of final network connectivity as well as induced electroosmosis were clarified. In addition, we propose a novel PEG gel electrophoresis strategy for separation of DNA fragments and low molecular weight compounds by doping graphene oxide (GO) into tetra-PEG gel. The results show that the addition of GO into PEG gel significantly improved the separation resolution of DNA fragments and considerably decreased electrophoretic mobility of small charged dyes ethidium bromide and rhodamine 6G. These results provide promising potential for graphene and its derivate utilized in various electrophoresis techniques for separation and detection of DNA fragments and other charged molecules.

聚合物网络中带电溶质的迁移率受扩散和电驱动迁移的控制，后者在凝胶电泳中过度使用。但是，由于影响迁移的结构参数的数量的不可合理性，迁移的物理机制仍然尚不清楚，因此对溶质 - 矩阵相互作用尚无清晰的了解。在本论文中，我们将TETRA-PEG凝胶用作模型系统，并研究了结构参数对恒定电场条件下DNA分子迁移的影响。阐明了最终网络连通性以及诱导的电流的影响。此外，我们提出了一种新型的PEG凝胶电泳策略，用于通过掺杂氧化石墨烯（GO）将DNA片段和低分子量化合物分离成四形凝胶。结果表明，加入PEG凝胶可显着改善DNA片段的分离分辨率，并大大降低了小带电染料溴化物和若丹明6G的电泳迁移率。这些结果为石墨烯及其在各种电泳技术中使用的衍生物提供了有希望的潜力，用于分离和检测DNA片段和其他带电分子。

项目成果

Electrophoretic Mobility of Double-Stranded DNA in Polymer Solutions and Gels with Tuned Structures

• DOI: 10.1021/ma500661r
• 发表时间: 2014-05
• 期刊: Macromolecules
• 影响因子: 5.5
• 作者: Xiang Li;Kateryna Khairulina;U. Chung;T. Sakai

Electrophoretic mobility of semi-flexible double-stranded DNA in defect-controlled polymer networks: mechanism investigation and role of structural parameters

缺陷控制聚合物网络中半柔性双链 DNA 的电泳迁移率：机制研究和结构参数的作用

• DOI: 10.1063/1.4922367
• 发表时间: 2015
• 期刊: J. Chem. Phys.
• 作者: Khairulina;K; Li;X; Nishi;K; Shibayama;M; Chung;U; Sakai;T.
• 通讯作者: T.

Hydrogels with tuned electro-osmosys:potential model system to understand electro-kinetic transport in biological tissues

具有调谐电渗透系统的水凝胶：了解生物组织中动电传输的潜在模型系统

• DOI: 10.1039/c7tb00064b
• 发表时间: 2017
• 期刊: Journal of Materials Chemistry B
• 影响因子: 7
• 作者: Khairulina;K.;Chung;U.;Sakai;T.
• 通讯作者: T.

Graphene oxide PEG hydrogels as efficient dye absorbents

氧化石墨烯 PEG 水凝胶作为高效染料吸收剂

• 发表时间: 2016
• 作者: Khairulina Kateryna;Yuta Nishina;Ung-il Chung;Takamasa Sakai
• 通讯作者: Takamasa Sakai

Mobility of low molecular weight compounds and dsDNA in graphene oxide-PEG composite hydrogels

低分子量化合物和双链DNA在氧化石墨烯-PEG复合水凝胶中的迁移率

• 发表时间: 2017
• 作者: Khairulina Kateryna;Yuta Nishina;Ung-il Chung;Takamasa Sakai
• 通讯作者: Takamasa Sakai