Sentinel lymph node metastasis imaging by using nanoparticle carriers in an animal study
在动物研究中使用纳米颗粒载体进行前哨淋巴结转移成像
基本信息
- 批准号:21592565
- 负责人:
- 金额:$ 2.91万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:2009
- 资助国家:日本
- 起止时间:2009 至 2011
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
We propose a new approach using multimodality imaging to facilitate sentinel node biopsy examinations, in which radioactive and near infrared(NIR) fluorescent nanoparticles can depict deep situated sentinel nodes and fluorescent nodes, as well as simultaneously visualize anatomical configurations in the field of interest. For this reason we have developed polyamidoamine(PAMAM) coated silica nanoparticles loaded with technetium-99m(Tc-99m) and ICG. To test the feasibility and utility of this dual-modality imaging system we conducted animal studies. The mean diameter of PAMAM coated silica nanoparticles was 30-50 nm as evaluated by images of transmission electron microscopy and scanning electron microscopy. The distribution of the particle size was measured by a laser scattering particle size analyzer(SALD-7100, Shimadzu, Japan). The peak of the distribution was 30 nm in ethanol solution. The combined labeling with both Tc-99m and ICG-Sulfo-OSu was verified by thin layer chromatography, … More TLC, before each experiment. 0.1 ml of the nanoparticle solution(7.4 MBq, except for one rat ; with 3.7 MBq, and 1 micro-gramm ICG) was submucosally injected in the tongue of six male Wister rats. Lymphoscintigraphy(sequential static images, every 5 minutes) was performed for 6 rats for one hour immediately after injection. Scintigraphic images showed an increased accumulation of Tc-99m in the neck with a markedly increased uptake at the injection site. Single increased nodal uptake was seen in 4 of 6 rats in the final static images. After completion of lymphoscintigraphy, animals were euthanized. As skin incision was made and 19 lymph nodes were identified in the dissected necks of 6 rats. After lymph node excision, contact-radiography was performed which revealed 3 markedly increased uptake nodes and 3 weak uptake nodes. NIR fluorescence imaging was performed on all dissected necks. The PDE system(Hamamatsu Photonics Co, Japan) provided clear fluorescence images of lymph nodes in the neck with anatomical configurations real time. The intensity of fluorescence was arbitrarily evaluated and 6 lymph nodes were weak to strong, (+)~(+++), and other lymph nodes were evaluated as having no fluorescence. Increased radioactive nodes coincided with fluorescent nodes. Radioactivity of 15 excised lymph nodes in 4 rats was assayed with a gamma-well counter. The radioactivity comparison revealed a large difference between the high fluorescence intensity group(4 lymph nodes, mean%; 0.109±0.067) and thelow or no fluorescence intensity group(8 lymph nodes, mean%; 0.001±0.000), (p<0.05). TEM revealed that small black granules were localized and dispersed within the cytoplasm of macrophages in lymph nodes. The appearance of small sphericalgranules was restricted to macrophages but not other lymphatic cells. Although further studies are needed to determine the appropriate sensitivity and safety of thisdual imaging nanoparticles, the results may promise for a better target nodes detection by the dual modality method in sentinel node biopsy. Less
我们提出了一种使用多模态成像来促进前哨淋巴结活检检查的新方法,其中放射性和近红外(NIR)荧光纳米粒子可以描绘位于深处的前哨淋巴结和荧光节点,并同时可视化感兴趣领域的解剖结构。为此,我们开发了负载锝-99m(Tc-99m)和ICG的聚酰胺胺(PAMAM)涂层二氧化硅纳米粒子,以测试可行性和实用性。我们对这种双模态成像系统进行了动物研究,通过透射电子显微镜和扫描电子显微镜的图像评估,PAMAM 涂覆的二氧化硅纳米颗粒的平均直径为 30-50 nm。通过激光散射测量颗粒尺寸的分布。粒度分析仪(SALD-7100,Shimadzu,日本)在乙醇溶液中的分布峰为30 nm。 ICG-Sulfo-OSu 在每次实验前通过薄层色谱、TLC 进行验证,将 0.1 ml 纳米颗粒溶液(7.4 MBq,一只大鼠除外;3.7 MBq 和 1 微克 ICG)粘膜下注射。对六只雄性 Wister 大鼠的舌头进行淋巴闪烁扫描(连续静态图像,每 5 分钟一次),持续一小时。注射后立即显示 Tc-99m 在颈部的积累增加,在最终静态图像中,6 只大鼠中的 4 只观察到单个淋巴结吸收增加。进行皮肤切口后,在 6 只大鼠的颈部解剖中发现了 19 个淋巴结,在淋巴结切除后进行接触性放射检查,发现 3 个淋巴结明显增大。对所有解剖颈部的摄取节点和3个弱摄取节点进行NIR荧光成像,PDE系统(日本Hamamatsu Photonics Co)提供了颈部淋巴的实时解剖结构的清晰荧光图像,荧光强度是任意的。评估6个淋巴结从弱到强,(+)~(+++),其余淋巴结评估为无荧光,与15个切除淋巴结的荧光淋巴结一致。用伽玛井计数器对4只大鼠的淋巴结进行了检测,放射性比较显示高荧光强度组(4个淋巴结,平均值%;0.109±0.067)和低或无荧光强度组(8个淋巴结,平均%)之间存在很大差异。平均%;0.001±0.000),(p<0.05),TEM显示小黑色颗粒集中并分散在淋巴结中的巨噬细胞的细胞质内。小球形颗粒的出现仅限于巨噬细胞,而不是其他淋巴细胞,尽管需要进一步研究来确定这种双成像纳米颗粒的适当敏感性和安全性,但结果可能有望通过双模态方法在前哨中更好地检测目标节点。淋巴结活检。较少
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Sentinel node imaging of radioactive and near infrared fluorescent nanocarriers
放射性和近红外荧光纳米载体的前哨节点成像
- DOI:
- 发表时间:2010
- 期刊:
- 影响因子:0
- 作者:Tsuchimochi M; Hayama K; Kameta A; Toyama M; Sasagawa I; TsubokawaN
- 通讯作者:TsubokawaN
Dual-modality Imaging Using Radionuclide and Near-infrared Fluorescence Nanoparticles for Sentinel Lymph Node Biopsy : An Animal Study
使用放射性核素和近红外荧光纳米颗粒进行前哨淋巴结活检的双模态成像:动物研究
- DOI:
- 发表时间:2011
- 期刊:
- 影响因子:0
- 作者:Tsuchimochi;K.Hayama;A.Kameta;H.Yamaguchi;M.Toyama;I.Sasagawa;N.Tsubokawa
- 通讯作者:N.Tsubokawa
ナノ粒子を用いた99mTcと近赤外蛍光の複合イメージングによるセンチネルリンパ節生検の可能性
使用 99mTc 和纳米粒子近红外荧光联合成像进行前哨淋巴结活检的可能性
- DOI:
- 发表时间:2010
- 期刊:
- 影响因子:0
- 作者:土持眞; 羽山和秀; 亀田綾子; 外山三智雄; 笹川一郎; 坪川紀夫
- 通讯作者:坪川紀夫
核医学と近赤外蛍光の複合イメージングナノ粒子によるセンチネルリンパ節生検の可能性
使用核医学和近红外荧光联合成像纳米粒子进行前哨淋巴结活检的可能性
- DOI:
- 发表时间:2011
- 期刊:
- 影响因子:0
- 作者:土持眞; 羽山和秀; 亀田綾子
- 通讯作者:亀田綾子
核医学と近赤外蛍光の複合イメージングナノ粒子によるセンチネルリンパ節生検の可能性
使用核医学和近红外荧光联合成像纳米粒子进行前哨淋巴结活检的可能性
- DOI:
- 发表时间:2011
- 期刊:
- 影响因子:0
- 作者:土持眞; 羽山和秀; 亀田綾子; 諏江美樹子
- 通讯作者:諏江美樹子
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TSUCHIMOCHI Makoto其他文献
TSUCHIMOCHI Makoto的其他文献
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{{ truncateString('TSUCHIMOCHI Makoto', 18)}}的其他基金
Theranostic study for neck micro-metastasis
颈部微转移的治疗诊断研究
- 批准号:
15K11303 - 财政年份:2015
- 资助金额:
$ 2.91万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Affibody probes for near-infrared fluorescence imaging of cancer cells in sentinel lymph nodes
用于前哨淋巴结癌细胞近红外荧光成像的 Affibody 探针
- 批准号:
24593040 - 财政年份:2012
- 资助金额:
$ 2.91万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
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Research and development of a new tracer for the diagnosis and treatment in the sentinel lymph node of breast cancer
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