New treatment for cerebral infarction using infiltrating cells

利用浸润细胞治疗脑梗塞的新疗法

• 批准号: 20591687
• 负责人: KUMON Yoshiaki
• 金额: $ 2.66万
• 依托单位: Ehime University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20591687/
• 关键词: 脳梗塞; 浸潤細胞; 神経栄養因子; 治療; 脳虚血; マクロファージ; 神経保護因子

We found that macrophage-like cells accumulate in the ischemic core of a rat brain whose right middle cerebral artery was transiently occluded for 90 minutes. Many of these cells expressed Iba1, a marker of macrophages/microglias, as well as NG2 chondroitin sulfate proteoglycan (NG2), which has been recognized as a marker of oligodendrocyte progenitor cells. Such macrophage-like cells were termed BINCs (brain Iba1+/NG2+cells). BINCs were highly proliferative and their number peaked at 7 days post-reperfusion. Taken the various function of NG2, BINCs may be involved in not only phagocytosis of degenerated cells but also the healing and regeneration of lesion cores. 5-Fluorouracil (5FU) injection at 2 days post reperfusion markedly reduced the number of BINCs at 7 days post reperfusion, causing enlargement of necrotic volumes and frequent death of the rats. When isolated BINCs were transplanted into 5FU-aggravated ischemic lesion, the volume of the lesion was much reduced. Bone marrow-derived BINCs play a benefical role in ischemic brain lesions, at least in part, through secretion of neuroprotective factors.

我们发现，在右侧大脑中动脉短暂闭塞 90 分钟的大鼠大脑缺血核心中，巨噬细胞样细胞积聚。其中许多细胞表达 Iba1（巨噬细胞/小胶质细胞的标记物）以及 NG2 硫酸软骨素蛋白聚糖 (NG2)（已被认为是少突胶质细胞祖细胞的标记物）。这种巨噬细胞样细胞被称为 BINC（脑 Iba1+/NG2+ 细胞）。 BINCs 高度增殖，其数量在再灌注后 7 天达到峰值。考虑到NG2的多种功能，BINCs可能不仅参与退化细胞的吞噬作用，还参与病变核心的愈合和再生。再灌注后2天注射5-氟尿嘧啶(5FU)显着减少了再灌注后7天的BINCs数量，导致坏死体积扩大和大鼠频繁死亡。当分离的BINCs被移植到5FU加重的缺血病灶中时，病灶的体积大大缩​​小。骨髓来源的 BINC 至少部分通过分泌神经保护因子在缺血性脑损伤中发挥有益作用。

项目成果

BINCs(Brain Iba1+/NG2+ Cells)の脳梗塞巣への侵入機序の検討

BINCs（Brain Iba1+/NG2+ Cells）侵袭脑梗死病灶的机制研究

• 发表时间: 2011
• 作者: 鄭菜里;渡邉英昭;久門良明;大西丘倫;田中潤也
• 通讯作者: 田中潤也

Postoperative temporary neurological deficits in adults with moyamoya disease.

成人烟雾病患者术后暂时性神经功能缺损。

• 发表时间: 2008
• 作者: Ohue S; Kumon Y; Kohno K; Watanabe H; Iwata S; Ohnishi T
• 通讯作者: Ohnishi T

Iba+/NG2+ macrophage-like cells expressing a variety of neuroprotective factors ameliorate ischemic damage of the brain.

表达多种神经保护因子的 Iba /NG2 巨噬细胞样细胞可改善大脑缺血性损伤。

• 发表时间: 2010
• 作者: Smirkin A; Matsumoto H; Takahashi H; Inoue A; Tagawa M; Ohue S; Watanabe H; Yano H; Kumon Y; Ohnishi T; Tanaka J
• 通讯作者: Tanaka J

ラット虚血脳におけるケモカイン;フラクタルカインとMCP-1

大鼠缺血脑中的趋化因子 fractalkine 和 MCP-1

• 发表时间: 2010
• 作者: 鄭菜里;渡邉英昭;久門良明;大西丘倫;田中潤也
• 通讯作者: 田中潤也

Vasodilator-stimulated phosphoprotein (VASP) phosphorylation assay for platelet response to cilostazol.

血管扩张剂刺激磷蛋白（VASP）磷酸化测定血小板对西洛他唑的反应。

• 发表时间: 2010
• 作者: Yamanouchi J; Hato T; Niiya T; Nakagawa K; Kumon Y; Fujiwara H; Yakushijin Y; Yasukawa M
• 通讯作者: Yasukawa M