IMMUNE RESPONSE OF MOSQUITOES TO FILARIAL WORMS

蚊子对丝虫的免疫反应

• 批准号: 2061015
• 负责人: BRUCE MARTIN CHRISTENSEN
• 金额: $ 19.77万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 1983
• 资助国家: 美国
• 起止时间: 1983-04-01 至 1995-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2061015
• 关键词: Aedes; Wuchereria; autoradiography; blood cells; cats; cell population study; communicable disease transmission; disease vectors; dogs; electron microscopy; enzyme mechanism; enzyme substrate; epidemiology; filariasis; gel electrophoresis; gerbil /jird; hemolymph; high performance liquid chromatography; host organism interaction; immunity; laboratory rabbit; ligase; oxidoreductase; protein sequence; transaminases

Immune mechanisms operating in mosquitoes are capable of limiting or preventing the development of filarial worms within these vectors. Although melanotic encapsulation reactions are responsible for resistance in certain species of mosquitoes, parasites are often capable of developing in other closely related species or even other strains, of the same species. Why certain mosquito species respond effectively while others do not, or why a species can immunologically destroy one parasite species but not another, are questions of fundamental relevance to our understanding of vector-parasite compatibility. The objective of the proposed research is to elucidate immune recognition and effector mechanisms and their regulation in mosquitoes exposed to filarial worms. Various biochemical and molecular techniques will be used with the mosquitoes Aedes aegypti, Aedes trivittatus and the filarial worms Brugia malayi, B. pahangi and Dirofilaria immitis (1) quantitatively assess the role specific enzymes and substrates might play in the production of protein-polyphenol complexes required for the immune destruction of microfilariae in mosquitoes, (2) identify and characterize hemolymph proteins that are uniquely or preferentially expressed in mosquitoes during melanotic encapsulation reactions, (3) characterize functional differences in mosquito hemocytes using lectins and anti-hemocyte monoclonal antibodies, and (4) begin characterizing immune mechanisms operating in the Armigeres subalbatus-Brugia spp. vector-parasite system that allows the development of B. pahangi but results in the immune destruction of B. malayi. The proposed studies will provide an increased clarification of mechanisms of immune responses in mosquitoes and a greater insight into vector-parasite associations, thereby increasing our understanding of the epidemiology of mosquito-borne filariasis.

在蚊子中运行的免疫机制能够限制或 防止这些向量中丝状蠕虫的发展。 尽管黑色素封装反应是抗药性的 在某些蚊子中，寄生虫通常能够发展 在其他密切相关的物种甚至其他菌株中 物种。 为什么某些蚊子物种有效地反应而其他蚊子会做出反应 不是，或者为什么一个物种可以在免疫学上破坏一种寄生虫，而是 与我们的理解有关 向量 - 寄生虫兼容性。 拟议的研究的目的是 阐明免疫识别和效应机制及其 暴露于丝状蠕虫的蚊子中的调节。 各种生化 和分子技术将与蚊子埃及埃及一起使用 Aedes Trivittatus和丝状蠕虫Brugia Malayi，B。Pahangi和 Dirofilaria Immisis（1）定量评估特定酶的作用和 底物可能会在蛋白质 - 聚苯酚复合物的生产中发挥作用 蚊子在蚊子中免疫破坏所必需的，（2） 识别和表征独特或 在黑素封装过程中优先在蚊子中表达 反应，（3）表征蚊子血细胞的功能差异 使用凝集素和抗虫细胞单克隆抗体，（4）开始 表征在Armigeres中运行的免疫机制 亚albatus-brugia spp。允许开发的矢量寄生虫系统 B. pahangi，但导致马来语的免疫破坏。 这 拟议的研究将增加对机制的澄清 蚊子中的免疫反应，对载体 - 寄生虫有更大的了解 协会，从而增加了我们对流行病学的理解 蚊子传播的丝虫病。