Elucidation of penetration mechanisms of sensory nerve fibers on a basement membrane in atopic dermatitis and application to intractable pruritus

特应性皮炎基底膜感觉神经纤维穿透机制的阐明及其在顽固性瘙痒症中的应用

• 批准号: 20591354
• 负责人: TAKAMORI Kenji
• 金额: $ 2.91万
• 依托单位: Juntendo University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20591354/
• 关键词: 痒み; 知覚神経; アトピー性皮膚炎; 細胞外マトリックス; MMP-2; MMP-8; 難治性痒み; 知覚神経線維; 真皮; Sema3A; NGF; コラーゲン; 基底膜

This study was performed to investigate mechanisms of penetration of sensory nerve fibres into the basement membrane of the skin. A rat dorsal root ganglion neuron culture system consisting of Matrigel and a Boyden chamber containing a nerve growth factor (NGF) concentration gradient was used, and membrane-associated MMP-2 contributes to penetration of nerve fibres into Matrigel through modulation by axonal guidance molecules and/or extracellular matrix components. Another study was performed to investigate elongation mechanisms of dermal nerve fibers within interstitial collagen matrix. Our study using the neuron culture system suggested that MMP-8 is involved in sensory nerve growth within interstitial collagen matrix.

本研究旨在研究感觉神经纤维穿透皮肤基底膜的机制。使用由基质胶和含有神经生长因子（NGF）浓度梯度的博伊登室组成的大鼠背根神经节神经元培养系统，膜相关的MMP-2通过轴突引导分子的调节有助于神经纤维渗透到基质胶中， /或细胞外基质成分。另一项研究是为了研究间质胶原基质内真皮神经纤维的伸长机制。我们使用神经元培养系统的研究表明，MMP-8 参与间质胶原基质内的感觉神经生长。

项目成果

In vitro model for penetration of sensory nerve fibers on a Matrigel basement membrane : Implications for possible application to intractable pruritus

基质胶基底膜上感觉神经纤维穿透的体外模型：可能应用于顽固性瘙痒的启示

• 发表时间: 2009
• 作者: Tominaga M; Kamo A; Tengara S; Ogawa H; Takamori K
• 通讯作者: Takamori K

Histological characterization of cutaneous nerve fibers containing gastrin-releasing peptide in NC/Nga mice : an atopic dermatitis model

NC/Nga 小鼠含有胃泌素释放肽的皮肤神经纤维的组织学特征：特应性皮炎模型

• 发表时间: 2009
• 作者: Tominaga M; Ogawa H; Takamori K
• 通讯作者: Takamori K

Status of plasmapheresis for the treatment of toxic epidermal necrolysis in Japan.

日本血浆置换术治疗中毒性表皮坏死松解症的现状。

• 发表时间: 2008
• 作者: Yamada H; Tominaga M
• 通讯作者: Tominaga M

TEX101, a germ cell-marker glycoprotein, is associated with lymphocyte antigen 6 complex locus k within the mouse testis.

TEX101 是一种生殖细胞标记糖蛋白，与小鼠睾丸内的淋巴细胞抗原 6 复合基因座 k 相关。

• 发表时间: 2008
• 作者: Yoshitake H; Tsukamoto H; Maruyama
• 通讯作者: Maruyama

アトピー性皮膚炎のバリア機能異常と痒みの関係

特应性皮炎屏障功能异常与瘙痒的关系

• 发表时间: 2008
• 作者: 高森建二