Analysis of MHC restriction between hemopoietic stem cells and mesenchymal stem cells in humans and mice

人和小鼠造血干细胞和间充质干细胞之间MHC限制分析

• 批准号: 20591159
• 负责人: HISHA Hiriko
• 金额: $ 2.33万
• 依托单位: Kansai Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20591159/
• 关键词: 造血幹細胞; 間葉系幹細胞; MHC拘束性; MHC class I分子; 臍帯血; 自己免疫疾患モデルマウス; 顆粒球・マクロファージコロニー刺激因子; 神経細胞接着分子; 接着分子; 羊膜

Engraftment of umbilical cord blood-derived hemopoietic stem cells (HSC) into SCID mice was facilitated when mesenchymal stem cells (MSCs) derived from the amnion of the same fetus were co-grafted. It is likely that G-CSF contributes to some extents to the preferential hemopoiesis-supporting ability of the MHC-matched MSCs. A higher proliferation of mouse HSCs was observed in co-culture system of HSCs expressing a high level of MHC class I molecule with MHC-matched MSCs than in that of HSCs expressing a low level of MHC class I molecule. Moreover, the abnormal proliferation (no MHC restriction) of HSCs in autoimmune-prone mice was attributed to increased expression of CD56 molecule on the HSC after the onset of autoimmune diseases.

当来自同一胎儿羊膜的间充质干细胞（MSC）共移植时，促进了脐带血来源的造血干细胞（HSC）植入SCID小鼠体内。 G-CSF可能在一定程度上有助于MHC匹配的MSC的优先造血支持能力。在表达高水平MHC I类分子的HSC与MHC匹配的MSC的共培养系统中观察到小鼠HSC的增殖高于表达低水平MHC I类分子的HSC。此外，在易患自身免疫性疾病的小鼠中，HSC 的异常增殖（无 MHC 限制）归因于自身免疫性疾病发作后 HSC 上 CD56 分子表达的增加。

项目成果

Human hemopoiesis-supporting capacity of mouse mesenchymal stem cell line : crucial role of neural cell adhesion molecule (NCAM)

小鼠间充质干细胞系的人类造血支持能力：神经细胞粘附分子（NCAM）的关键作用

• 发表时间: 2010
• 作者: Hiroko Hisha

Repeated infusions of donor blood, BMCs and PBSCs can restore early hematopoiesis after allogenic BMT

反复输注供血、BMC和PBSC可恢复同种异体BMT后的早期造血功能

• 发表时间: 2009
• 作者: 李清

Contribution of neural cell adhesion molecule (NCAM) to monkey hemopoietic systems (III)

神经细胞粘附分子（NCAM）对猴造血系统的贡献（三）

• 发表时间: 2008
• 作者: 加藤順子

Transformation potential of bone marrow stromal cells into undifferentiated high grade pleomorphic sarcoma

骨髓基质细胞向未分化高级别多形性肉瘤的转化潜力

• 发表时间: 2010
• 作者: Qing Li

Hemopoiesis-supporting capacity of mouse mesenchymal stem cell line : Crucial role of neural cell adhesion molecule (NCAM).

小鼠间充质干细胞系的造血支持能力：神经细胞粘附分子（NCAM）的关键作用。

• 发表时间: 2010
• 作者: Hiroko Hisha; Xiaoli Wang; Junko Kato; Tomomi Mizokami; Qing Li; Hideharu Kanzaki; Susumu Ikehara
• 通讯作者: Susumu Ikehara