Elucidation of onset mechanism of amelogenesis imperfecta (AI) using a biomineralization model

使用生物矿化模型阐明釉质生成不全（AI）的发病机制

• 批准号: 20390522
• 负责人: KOTARO Tanimoto
• 金额: $ 6.16万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20390522/
• 关键词: エナメル蛋白; バイオミネラリゼーション; エナメル質形成不全症; アメロゲニン; ハイドロキシアパタイト

The purpose of this study was to investigate the effects of mutation of amelgenin, an enamel matrix protein, on the enamel crystal formation, and to elucidate of onset mechanism of amelogenesis imperfecta (AI).Wild-type recombinant human full-length amelogenin and mutated recombinant human full-length ameogenin (P41T) were synthesized. There was a difference in the aggregation ability in solution at neutral pH (pH 7.4) between the wild-type and mutated full-length amelogenins. In addition, recombinant human MMP20 wa synthesized, and the digestion of recombinant human full-length amelogenin by the MMP20 was investigated. The digestion of mutated recombinant human full-length amelogenin was delayed as compared with that in wild-type amelogenin.From these results, it was strongly suggested that a mutation of amelogenin may induce the abnormality of enamel structure during tooth formation.

本研究的目的是探讨釉质基质蛋白釉原蛋白突变对釉质晶体形成的影响，并阐明釉质形成不全症（AI）的发病机制。野生型重组人全长釉原蛋白和突变体合成了重组人全长ameogenin (P41T)。野生型和突变型全长牙釉蛋白在中性pH（pH 7.4）溶液中的聚集能力存在差异。另外，合成了重组人MMP20，并研究了MMP20对重组人全长牙釉蛋白的消化。与野生型牙釉蛋白相比，突变的重组人全长牙釉蛋白的消化被延迟。这些结果强烈表明牙釉蛋白的突变可能导致牙齿形成过程中牙釉质结构的异常。

项目成果

Amelogenin Modulates the Proliferation of Human Mesenchymal Stem Cells through the MAPK-ERK Pathway

Amelogenin 通过 MAPK-ERK 途径调节人间充质干细胞的增殖

• 发表时间: 2010
• 作者: Huang YC; Tanimoto K; Tanne Y; Kamiya T; Ohkuma S; Inubushi T; Kunimatsu R; Yoshioka M; Michida M; Mitsuyoshi T; Yoshimi Y; Kato Y; Tanne K
• 通讯作者: Tanne K

Effecs of amelogenin on the proliferation of mesenchymak stem cells

釉原蛋白对间充质干细胞增殖的影响

• 发表时间: 2010
• 作者: 黄郁〓; 谷本幸太郎; 丹根由起; 神谷貴志; 犬伏俊博; 吉岡基子; 道田将彦; 笹本智子; 吉見友希; 岩渕泰憲; 丹根一夫
• 通讯作者: 丹根一夫

Comparative properties of recombinant human and bovine matrix metalloproteinase-20.

重组人和牛基质金属蛋白酶-20 的特性比较。

• 发表时间: 2008
• 作者: Zhu L.

Effects of recombinant human amelogenin on the proliferation of cementoblasts and periodontal ligament cells

重组人牙釉蛋白对成牙骨质细胞和牙周膜细胞增殖的影响

• 发表时间: 2009
• 作者: 国松亮; 谷本幸太郎; 田中伸明; 丹根由起; 神谷貴志; 大熊暁; 犬伏俊博; 黄郁〓; 吉岡基子; 廣瀬尚人; 光吉智美; 吉見友希; 宮内睦美; 高田隆; 丹根一夫
• 通讯作者: 丹根一夫

Amelogenin enhances the proliferation of cementoblast lineage cells

釉原蛋白增强成牙骨质细胞谱系细胞的增殖

• 发表时间: 2011
• 作者: Kunimatsu R; Tanimoto K; Tanne Y; Kamiya T; Ohkuma S; Huang YC; Yoshimi Y; Miyauchi M; Takata T; Tanne K.
• 通讯作者: Tanne K.