Structures Derived from Mass Spectrometric Peptide Fragmentation Pathways Explored by Combined Experimental and Theoretical Approaches

通过实验和理论相结合的方法探索质谱肽断裂途径衍生的结构

• 批准号: 5423797
• 负责人: Professor Dr. Sandor Suhai
• 金额: --
• 依托单位: Forschungsschwerpunkt Funktionelle und strukturelle Genomforschung
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2004
• 资助国家: 德国
• 起止时间: 2003-12-31 至 2008-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5423797?language=en
• 关键词: Structures; Derived; Mass; Spectrometric; Peptide

Mass spectrometry (MS) has recently become one of the major analytical tools used in biosciences and constitutes one of the most important ingredients of proteomics. The evolution of MS has involved the introduction of ionization techniques applicable to large bio-molecules, improving the mass range and resolution of mass spectrometers, and development of new algorithms for handling the extensive data produced. Tandem mass spectrometry (MS/MS) is one of the most important MS techniques applied to proteins and peptides which involves creation and dissociation of ions of the present proposal are determination of the structure of peptidce ions and fragments by using experimental and computational approach to investigate the MS/MS spectra of intities of peptice libraries synthesized by using combinatorial chemistry techniques. These investigations will cover the most important peptide classes (for example tryptic peptides) occurring in everyday practice. Our studies will lead to detailed understanding of the structure and reactions of peptide ions and will provide 'sequencing rules' which will improve the available protein identification algorithms used in MS-based proteomics.

质谱（MS）最近已成为生物科学中使用的主要分析工具之一，并构成蛋白质组学最重要的组成部分之一。 MS 的发展涉及引入适用于大生物分子的电离技术、提高质谱仪的质量范围和分辨率以及开发用于处理所产生的大量数据的新算法。串联质谱 (MS/MS) 是应用于蛋白质和肽的最重要的 MS 技术之一，涉及离子的产生和解离，本提案是通过使用实验和计算方法来研究确定肽离子和片段的结构使用组合化学技术合成的肽库的 MS/MS 谱图。这些研究将涵盖日常实践中最重要的肽类别（例如胰蛋白酶肽）。我们的研究将导致对肽离子的结构和反应的详细了解，并将提供“测序规则”，这将改进基于 MS 的蛋白质组学中使用的可用蛋白质识别算法。