The Bioimaging Core

生物成像核心

基本信息

批准号：
10612971
负责人：
Kenneth P Olive
金额：
$ 21.65万
依托单位：
COLUMBIA UNIVERSITY HEALTH SCIENCES
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-04-30 至 2027-03-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10612971
关键词：
3-Dimensional Animals Basic Science Bioinformatics Biology Cell Culture Techniques Cell physiology Cells Cellular Structures Cellular biology Chemistry Clinical Collaborations Complex Data Development Digestive Physiology Digestive System Disorders Disease Doctor of Philosophy Education Educational workshop Emerging Technologies Engineering Ensure Epithelial Cells Epithelium Evaluation Feedback Fees Fibroblasts Fibrosis Fluorescence Fluorescence Microscopy Funding Future Gastrointestinal Motility Goals Health Herbert Irving Comprehensive Cancer Center Histology Homeostasis Human Human Resources Image Imaging Device Imaging technology Immune In Vitro Individual Inflammation Intervention Laboratories Leadership Link Lipids Liver diseases Magnetic Resonance Imaging Measurement Microbe Microscope Microscopy Mission Mus Natural regeneration Neoplasms Nerve Olives - dietary Optics Organ Organizational Efficiency Organoids Peptides Pharmaceutical Preparations Proteins Protocols documentation Qualifying Quality Control Research Research Personnel Research Support Resolution Resource Sharing Resources Science Scientist Services Signal Pathway Specimen Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Surveys Technology Time Tissues Training Training and Education United States National Institutes of Health Universities X-Ray Computed Tomography animal imaging bioimaging cell motility clinical imaging cost experience fluorescence imaging high resolution imaging human imaging imaging capabilities imaging modality imaging platform in vivo in vivo imaging innovation innovative technologies instrumentation mass spectrometric imaging member microCT microbiota multi-photon multimodal data multiple datasets nanometer new technology novel operation optical imaging patient derived xenograft model programs reflectance confocal microscopy response single cell analysis single-cell RNA sequencing success superresolution microscopy technology platform tool transcriptomics ultra high resolution ultrasound

项目摘要

SUMMARY The Bioimaging Core (BIC) in the Columbia University Digestive and Liver Disease Research Center (CU- DLDRC) will make essential contributions for the understanding and treatment of digestive diseases. State-of- the-art imaging can resolve the complex interactions between cells, organs, metabolites and microbiota that regulate digestive health and disease. However, the cost and expertise of maintaining and operating complex imaging platforms are beyond the financial and technical means of most individual laboratories. The overall goal of the BIC is to provide CU-DLDRC members with state-of-the-art imaging platforms to study mechanisms of digestive health and disease in small animals and human biospecimens in vitro and in vivo, providing resolutions ranging from nanometers to centimeters. The BIC is organized into two units with high-end digestive-focused imaging capabilities backed by expert technical assistance, each led by a highly qualified director with longstanding experience in imaging and core administration. The Microscopy Unit, overseen by BIC Co-Director Liza Pon, provides access to a sophisticated suite of microscopes, including single, multiphoton and spinning disk confocal, super-resolution and total internal reflection fluorescence microscopy, which allow rapid and high- resolution imaging of living or fixed digestive tissue specimens. The Small Animal Imaging Unit, overseen by BIC director Ken Olive, provides access to high-end magnetic resonance imaging, ultrasound, X-ray computed tomography, and optical imaging instruments for imaging of digestive organs and their functions in small animals. In addition, the BIC will develop new digestive-focused imaging modalities, including (i) imaging mass spectrometry with CU-DLDRC member Brent Stockwell for high resolution imaging of metabolites in digestive tissue; and (ii) SCAPE imaging, a novel meso-scale optical platform based on endogenous tissue fluorescence, engineered by CU-DLDRC member Elizabeth Hillman. The Small Animal Imaging unit will also develop novel applications specific for the assessment of gastrointestinal motility, fibrosis, inflammation, tracking of engineered microbes, and in vivo measurement of metabolites. The BIC will contribute to the overall mission of the CU- DLDRC through the following interrelated Aims: To analyze cellular structures and processes that regulate epithelial health in digestive tissues via microscopy (Aim 1); to provide in vivo small animal imaging technologies for the study of digestive physiology and disease (Aim 2); and to provide hands-on training and education in advanced digestive imaging applications (Aim 3). BIC will be a highly utilized resource with 43 out of the 49 CU- DLDRC members (88%) planning to use its services. Via multicore workflows, BIC will closely link to the other CU-DLDRC biomedical cores, thus providing CU-DLDRC members with easy access to imaging clinical biospecimens and organoids, or combining their imaging with advanced bioinformatics analyses. In sum, BIC will benefit the research of basic and clinical CU-DLDRC members and provide significant value added.

概括哥伦比亚大学消化和肝脏疾病研究中心 (CU- DLDRC）将为消化系统疾病的理解和治疗做出重要贡献。状态- 最先进的成像技术可以解决细胞、器官、代谢物和微生物群之间复杂的相互作用调节消化系统健康和疾病。然而，维护和运营复杂的成本和专业知识成像平台超出了大多数个体实验室的财务和技术能力。总体目标 BIC 的目标是为 CU-DLDRC 成员提供最先进的成像平台来研究小动物和人类生物样本的体外和体内消化健康和疾病，提供解决方案范围从纳米到厘米。 BIC 分为两个单元，以高端消化为重点成像能力得到专家技术协助的支持，每一位技术协助均由一位高素质的主管领导在成像和核心管理方面拥有长期经验。显微镜科，由 BIC 联合主任监督 Liza Pon，提供了一套复杂的显微镜，包括单光子、多光子和旋转显微镜圆盘共焦、超分辨率和全内反射荧光显微镜，可实现快速、高精度活体或固定消化组织标本的分辨率成像。小动物成像中心，由 BIC 监管总监 Ken Olive，提供高端磁共振成像、超声波、X 射线计算断层扫描和光学成像仪器，用于对小动物的消化器官及其功能进行成像。此外，BIC 将开发新的消化聚焦成像模式，包括 (i) 质量成像与 CU-DLDRC 成员 Brent Stockwell 合作进行光谱测定，对消化道中的代谢物进行高分辨率成像组织; (ii) SCAPE 成像，一种基于内源组织荧光的新型介观光学平台，由 CU-DLDRC 成员 Elizabeth Hillman 设计。小动物成像装置也将开发新颖的专门用于评估胃肠道运动、纤维化、炎症、跟踪工程化的应用微生物和代谢物的体内测量。 BIC 将为 CU 的总体使命做出贡献 DLDRC 通过以下相互关联的目标：分析调节细胞结构和过程通过显微镜观察消化组织上皮的健康状况（目标 1）；提供小动物活体成像技术用于消化生理学和疾病的研究（目标 2）；并提供实践培训和教育先进的消化成像应用（目标 3）。 BIC 将成为一种高度利用的资源，49 个 CU 中有 43 个 DLDRC 成员 (88%) 计划使用其服务。通过多核工作流程，BIC 将与其他工作流程紧密相连 CU-DLDRC 生物医学核心，从而为 CU-DLDRC 成员提供轻松访问影像临床的机会生物样本和类器官，或将其成像与先进的生物信息学分析相结合。总而言之，BIC 将有利于 CU-DLDRC 成员的基础和临床研究，并提供显着的附加值。