The Bioimaging Core

生物成像核心

• 批准号: 10612971
• 负责人: Kenneth P Olive
• 金额: $ 21.65万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-04-30 至 2027-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10612971
• 关键词: 3-Dimensional; Animals; Basic Science; Bioinformatics; Biology; Cell Culture Techniques; Cell physiology; Cells; Cellular Structures; Cellular biology; Chemistry; Clinical; Collaborations; Complex; Data; Development; Digestive Physiology; Digestive System Disorders; Disease; Doctor of Philosophy; Education; Educational workshop; Emerging Technologies; Engineering; Ensure; Epithelial Cells; Epithelium; Evaluation; Feedback; Fees; Fibroblasts; Fibrosis; Fluorescence; Fluorescence Microscopy; Funding; Future; Gastrointestinal Motility; Goals; Health; Herbert Irving Comprehensive Cancer Center; Histology; Homeostasis; Human; Human Resources; Image; Imaging Device; Imaging technology; Immune; In Vitro; Individual; Inflammation; Intervention; Laboratories; Leadership; Link; Lipids; Liver diseases; Magnetic Resonance Imaging; Measurement; Microbe; Microscope; Microscopy; Mission; Mus; Natural regeneration; Neoplasms; Nerve; Olives - dietary; Optics; Organ; Organizational Efficiency; Organoids; Peptides; Pharmaceutical Preparations; Proteins; Protocols documentation; Qualifying; Quality Control; Research; Research Personnel; Research Support; Resolution; Resource Sharing; Resources; Science; Scientist; Services; Signal Pathway; Specimen; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Surveys; Technology; Time; Tissues; Training; Training and Education; United States National Institutes of Health; Universities; X-Ray Computed Tomography; animal imaging; bioimaging; cell motility; clinical imaging; cost; experience; fluorescence imaging; high resolution imaging; human imaging; imaging capabilities; imaging modality; imaging platform; in vivo; in vivo imaging; innovation; innovative technologies; instrumentation; mass spectrometric imaging; member; microCT; microbiota; multi-photon; multimodal data; multiple datasets; nanometer; new technology; novel; operation; optical imaging; patient derived xenograft model; programs; reflectance confocal microscopy; response; single cell analysis; single-cell RNA sequencing; success; superresolution microscopy; technology platform; tool; transcriptomics; ultra high resolution; ultrasound; 3-Dimensional; Animals; Basic Science; Bioinformatics; Biology; Cell Culture Techniques; Cell physiology; Cells; Cellular Structures; Cellular biology; Chemistry; Clinical; Collaborations; Complex; Data; Development; Digestive Physiology; Digestive System Disorders; Disease; Doctor of Philosophy; Education; Educational workshop; Emerging Technologies; Engineering; Ensure; Epithelial Cells; Epithelium; Evaluation; Feedback; Fees; Fibroblasts; Fibrosis; Fluorescence; Fluorescence Microscopy; Funding; Future; Gastrointestinal Motility; Goals; Health; Herbert Irving Comprehensive Cancer Center; Histology; Homeostasis; Human; Human Resources; Image; Imaging Device; Imaging technology; Immune; In Vitro; Individual; Inflammation; Intervention; Laboratories; Leadership; Link; Lipids; Liver diseases; Magnetic Resonance Imaging; Measurement; Microbe; Microscope; Microscopy; Mission; Mus; Natural regeneration; Neoplasms; Nerve; Olives - dietary; Optics; Organ; Organizational Efficiency; Organoids; Peptides; Pharmaceutical Preparations; Proteins; Protocols documentation; Qualifying; Quality Control; Research; Research Personnel; Research Support; Resolution; Resource Sharing; Resources; Science; Scientist; Services; Signal Pathway; Specimen; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Surveys; Technology; Time; Tissues; Training; Training and Education; United States National Institutes of Health; Universities; X-Ray Computed Tomography; animal imaging; bioimaging; cell motility; clinical imaging; cost; experience; fluorescence imaging; high resolution imaging; human imaging; imaging capabilities; imaging modality; imaging platform; in vivo; in vivo imaging; innovation; innovative technologies; instrumentation; mass spectrometric imaging; member; microCT; microbiota; multi-photon; multimodal data; multiple datasets; nanometer; new technology; novel; operation; optical imaging; patient derived xenograft model; programs; reflectance confocal microscopy; response; single cell analysis; single-cell RNA sequencing; success; superresolution microscopy; technology platform; tool; transcriptomics; ultra high resolution; ultrasound

SUMMARY The Bioimaging Core (BIC) in the Columbia University Digestive and Liver Disease Research Center (CU- DLDRC) will make essential contributions for the understanding and treatment of digestive diseases. State-of- the-art imaging can resolve the complex interactions between cells, organs, metabolites and microbiota that regulate digestive health and disease. However, the cost and expertise of maintaining and operating complex imaging platforms are beyond the financial and technical means of most individual laboratories. The overall goal of the BIC is to provide CU-DLDRC members with state-of-the-art imaging platforms to study mechanisms of digestive health and disease in small animals and human biospecimens in vitro and in vivo, providing resolutions ranging from nanometers to centimeters. The BIC is organized into two units with high-end digestive-focused imaging capabilities backed by expert technical assistance, each led by a highly qualified director with longstanding experience in imaging and core administration. The Microscopy Unit, overseen by BIC Co-Director Liza Pon, provides access to a sophisticated suite of microscopes, including single, multiphoton and spinning disk confocal, super-resolution and total internal reflection fluorescence microscopy, which allow rapid and high- resolution imaging of living or fixed digestive tissue specimens. The Small Animal Imaging Unit, overseen by BIC director Ken Olive, provides access to high-end magnetic resonance imaging, ultrasound, X-ray computed tomography, and optical imaging instruments for imaging of digestive organs and their functions in small animals. In addition, the BIC will develop new digestive-focused imaging modalities, including (i) imaging mass spectrometry with CU-DLDRC member Brent Stockwell for high resolution imaging of metabolites in digestive tissue; and (ii) SCAPE imaging, a novel meso-scale optical platform based on endogenous tissue fluorescence, engineered by CU-DLDRC member Elizabeth Hillman. The Small Animal Imaging unit will also develop novel applications specific for the assessment of gastrointestinal motility, fibrosis, inflammation, tracking of engineered microbes, and in vivo measurement of metabolites. The BIC will contribute to the overall mission of the CU- DLDRC through the following interrelated Aims: To analyze cellular structures and processes that regulate epithelial health in digestive tissues via microscopy (Aim 1); to provide in vivo small animal imaging technologies for the study of digestive physiology and disease (Aim 2); and to provide hands-on training and education in advanced digestive imaging applications (Aim 3). BIC will be a highly utilized resource with 43 out of the 49 CU- DLDRC members (88%) planning to use its services. Via multicore workflows, BIC will closely link to the other CU-DLDRC biomedical cores, thus providing CU-DLDRC members with easy access to imaging clinical biospecimens and organoids, or combining their imaging with advanced bioinformatics analyses. In sum, BIC will benefit the research of basic and clinical CU-DLDRC members and provide significant value added.

概括 哥伦比亚大学消化和肝病研究中心的生物成像核心（BIC）（CU- DLDRC）将为理解和治疗消化系统疾病做出重要贡献。最新 ART成像可以解决细胞，器官，代谢产物和微生物群之间的复杂相互作用， 调节消化健康和疾病。但是，维护和运营复杂的成本和专业知识 成像平台超出了大多数个人实验室的财务和技术手段。总体目标 BIC的内容是为Cu-DLDRC成员提供最新的成像平台，以研究 小动物的消化健康和疾病在体外和体内生物测量，提供决议 从纳米到厘米。 BIC被组织成两个以高端消化为中心的单元 由专家技术援助支持的成像功能，每位能力由一位高素质的导演领导 在成像和核心管理方面的长期经验。显微镜单元，由BIC共同导演监督 liza pon，可访问一套复杂的显微镜，包括单个，多光子和旋转 磁盘共焦，超分辨率和总内反射荧光显微镜，可快速和高 生命或固定消化组织标本的分辨率成像。小动物成像单元，由BIC监督 导演肯·奥利弗（Ken Olive）提供了对高端磁共振成像的访问权限，超声，X射线计算 断层扫描和光学成像仪器，用于成像消化器官及其在小动物中的功能。 此外，BIC将发展新的以消化为中心的成像方式，包括（i）成像质量 具有Cu-DLDRC成员Brent Stockwell的光谱法，用于消化中代谢物的高分辨率成像 组织; （ii）Scape Imaging是一种基于内源性组织荧光的新型中尺度光学平台， 由CU-DLDRC成员Elizabeth Hillman设计。小型动物成像单元也将发展出小说 用于评估胃肠运动，纤维化，炎症，工程过程的应用 微生物和代谢物的体内测量。 BIC将有助于Cu-的整体任务 通过以下相互关联的目的DLDRC：分析调节细胞结构和过程 通过显微镜检查消化组织中的上皮健康（AIM 1）；提供体内小动物成像技术 用于研究消化生理和疾病（AIM 2）；并提供动手培训和教育 高级消化成像应用（AIM 3）。 BIC将是高度利用的资源，其中49立方米中有43个 DLDRC成员（88％）计划使用其服务。通过多核工作流，BIC将紧密链接到另一个 Cu-DLDRC生物医学核心，从而为Cu-dldrc成员提供了容易访问临床成像的 生物测量和器官，或将成像与先进的生物信息学分析相结合。总而言之，比克 将有益于基本和临床CU-DLDRC成员的研究，并提供大量的增值。