Analysis of hydrogenase genes for the ecological elucidation of hydrogen-producing bacterial community in paddy field soil

氢化酶基因分析用于稻田土壤产氢细菌群落生态学解析

• 批准号: 24780318
• 负责人: WATANABE Takeshi
• 金额: $ 2.91万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24780318/
• 关键词: 水素生成; ヒドロゲナーゼ; 水田; 群集構造解析; 水田土壌; FeFeヒドロゲナーゼ

In this study, molecular biological analysis of hydA gene, encoding [FeFe]-hydrogenase related to hydrogen production, was carried out to reveal diversity and dynamics of the hydrogen-producing bacterial community in paddy field soil. This study showed inhabitant of diverse bacteria possessing [FeFe]-hydrogenase and their stable community structures in the paddy field soil. However, hydA-transcribing bacterial groups changed significantly during the anaerobic decomposition of organic materials in the soil, indicating that the hydrogen-producing bacterial groups and their hydrogen-producing activity varied depending on the soil conditions.

本研究对编码与产氢相关的[FeFe]-氢化酶的hydA基因进行了分子生物学分析，以揭示稻田土壤产氢细菌群落的多样性和动态。本研究表明，稻田土壤中存在着多种具有[FeFe]-氢化酶的细菌及其稳定的群落结构。然而，在土壤中有机物质的厌氧分解过程中，hydA转录细菌群发生了显着变化，这表明产氢细菌群及其产氢活性根据土壤条件而变化。

项目成果

Changes in community structure of methanogenic archaea brought about by water-saving practice in paddy field soil

• DOI: 10.1016/j.soilbio.2012.11.022
• 发表时间: 2013-03
• 期刊: Soil Biology & Biochemistry
• 影响因子: 9.7
• 作者: Takeshi Watanabe;Y. Hosen;R. Agbisit;L. Llorca;N. Katayanagi;S. Asakawa;M. Kimura

Hydrogen-producing bacterial community in paddy field soils estimated by [FeFe] hydrogenase gene

[FeFe]氢化酶基因估算稻田土壤产氢细菌群落

• 发表时间: 2012
• 作者: Yoko Suzuki;Masafumi Shimizu and Mitsuro Hyakumachi;Ryuko Baba・Makoto Kimura・Susumu Asakawa・Takeshi Watanabe
• 通讯作者: Ryuko Baba・Makoto Kimura・Susumu Asakawa・Takeshi Watanabe

Effect of winter-flooding on methanogenic archaeal community structure in paddy field under organic farming

• DOI: 10.1080/00380768.2012.726598
• 发表时间: 2012-10
• 期刊: Soil Science and Plant Nutrition
• 影响因子: 2
• 作者: Dongyan Liu;Chika Suekuni;K. Akita;Toyoaki Ito;M. Saito;Takeshi Watanabe;M. Kimura;S. Asakawa

生態学的手法による水田土壌のメタン生成古細菌の動態と多様性に

利用生态方法研究水稻土中产甲烷古菌的动态和多样性

• 发表时间: 2013
• 作者: 横山 咲;中村 進一;服部 浩之;頼 泰樹;小林沙織;渡邉健史
• 通讯作者: 渡邉健史

水田土壌に形成されたコロニー中の鉄酸化細菌の分子系統学的解析

水稻土中铁氧化菌菌落的分子系统发育分析

• 发表时间: 2012
• 作者: 渡邉健史・隅田裕明・M.D. Nhut・矢野勝也・浅川晋・木村眞人