Elucidation of essential growth factors for mouse spermatogenesis using organ culture method.

使用器官培养方法阐明小鼠精子发生的必需生长因子。

• 批准号: 24770216
• 负责人: SATO Takuya
• 金额: $ 3万
• 依托单位: Yokohama City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24770216/
• 关键词: 精子形成; 器官培養; 精子幹細胞; 男性不妊; Stem cell factor; 不妊治療; CSF1

In the present study, using a testis tissue culture method, we challenged to treat a spermatogenic defect mutant without any genetic manipulations. The Sl/Sld mouse has only a few primitive spermatogonia as germ cells in the testis, lacking any sign of spermatogenesis, due to mutations of the c-Kit ligand gene (Kitl) which cause the loss of membrane-bound-type KITL from the surface of Sertoli cells. We succeeded in inducing the complete spermatogenesis of Sl/Sld mutant mice by culturing their testis tissues in culture media containing KITL and colony stimulating factor-1 (CSF-1).To elucidate the mechanism of CSF-1's effect on spermatogenesis, we performed transplantation experiment of spermatogonial stem cells derived from CSF-1 receptor knock out mouse.

在本研究中，使用睾丸组织培养方法，我们挑战了无需任何遗传操作而无需治疗精子缺陷突变体。 SL/SLD小鼠仅具有少数原始的精子症作为睾丸中的生殖细胞，由于C-KIT配体基因（KITL）的突变导致膜结合膜型基特尔的损失，因此缺乏精子生成的迹象。 We succeeded in inducing the complete spermatogenesis of Sl/Sld mutant mice by culturing their testis tissues in culture media containing KITL and colony stimulating factor-1 (CSF-1).To elucidate the mechanism of CSF-1's effect on spermatogenesis, we performed transplantation experiment of spermatogonial stem cells derived from CSF-1 receptor knock out mouse.

项目成果

In vitro spermatogenesis in cultured adult mouse testes.

培养的成年小鼠睾丸的体外精子发生。

• 发表时间: 2013
• 作者: Sato T.;Katagiri K.;Yokonishi T.;Komeya M.;Kubota Y.;Ogawa
• 通讯作者: Ogawa

In vitro spermatogenesis in cultured adult mouse testes

培养的成年小鼠睾丸的体外精子发生

• 发表时间: 2013
• 作者: Sato T.;Katagiri K.;Yokonishi T.;Komeya M.;Kubota Y.;Ogawa
• 通讯作者: Ogawa

新生仔マウス精巣からの試験管内精子産生

新生小鼠睾丸的体外精子生产

• 发表时间: 2013
• 期刊: BIO Clinica
• 作者: Shiura H;Ikeda R;Lee J;Sato T;Ogonuki N;Hirose M;Ogura A;Ogawa T;Abe K.;佐藤卓也・小川毅彦;佐藤卓也・小川毅彦
• 通讯作者: 佐藤卓也・小川毅彦

目で見る生殖幹細胞-精子幹細胞

视觉生殖干细胞 - 精子干细胞

• 发表时间: 2013
• 期刊: HORMONE FRONTIER IN GYNECOLOGY
• 作者: Shiura H;Ikeda R;Lee J;Sato T;Ogonuki N;Hirose M;Ogura A;Ogawa T;Abe K.;佐藤卓也・小川毅彦
• 通讯作者: 佐藤卓也・小川毅彦

器官培養法をもちいたc-Kit ligand変異不妊マウスの精子形成誘導

器官培养法诱导c-Kit配体突变不育小鼠精子发生

• 发表时间: 2013
• 作者: T.Sato;T.Yokonishi;M.Komeya;K.Katagiri;Y.Kubota;S.Matoba;N.Ogonuki;A.Ogura;S.Yoshida and T.Ogawa;佐藤卓也・他9名
• 通讯作者: 佐藤卓也・他9名