Evaluation of in situ tissue enginnering of the middle ear mucosa

中耳粘膜原位组织工程评价

• 批准号: 24791787
• 负责人: AKIYAMA Naotaro
• 金额: $ 2.75万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24791787/
• 关键词: 中耳; 中耳粘膜上皮; 再生; 培養細胞; 中人粘膜上皮

Middle ear mucosa is essential in the maintenance of middle ear function. In the present study, we evaluated in situ tissue engineering for the mucosal regeneration in the rat middle ear.We prepared donor cells from SD-transgenic rats which expressed EGFP systemically. The cultured cells were encapsulated into the synthetic peptide hydrogel and transplanted into the middle ear of SD rats, where middle ear mucosa was eliminated surgically. At 7, 14, and 28 days after transplantation, the middle ear bullae with transplanted cells were collected and analyzed.In many cases, EGFP expressed cells were found on the surface of the bone of host-middle ear bullae and retained the character of epithelial cells immunohistochemically. Ultrastructural analysis revealed the adhesion junction like structure between adjacent cells.In conclusion, these findings suggested that transplanted donor cells had migrated into the host tissue and reorganized normal structure of the middle ear epithelial cells.

中耳粘膜对于维持中耳功能至关重要。在本研究中，我们评估了大鼠中耳中粘膜再生的原位组织工程。我们准备了来自SD-转基因大鼠的供体细胞，这些供体细胞系统地表达了EGFP。将培养的细胞封装在合成肽水凝胶中，并将其移植到SD大鼠的中耳中，其中中耳粘膜被外科手术消除。在移植后的7、14和28天，收集带有移植细胞的中耳小桶并进行分析。在许多情况下，在宿主中部耳朵的骨头表面发现EGFP表达的细胞，并保留了上皮性特征细胞通过免疫组织化学。超微结构分析揭示了相邻细胞之间的粘附连接类似结构。总而言，这些发现表明，移植的供体细胞已迁移到宿主组织中，并重新组织了中耳上皮细胞的正常结构。

项目成果

Otoendoscopic Examination and Micro-computed Tomography Image Analysis of a New Animal Model of Continuous Negative Pressure in the Middle Ear

新型中耳持续负压动物模型的耳内镜检查和显微计算机断层扫描图像分析

• 发表时间: 2013
• 作者: 穐山直太郎;福田智美;髙橋晴雄;小路武彦;Naotaro Akiyama
• 通讯作者: Naotaro Akiyama

ペプチドハイドロゲルを用いたラット中耳粘膜培養細胞による中耳粘膜再生の検討

使用肽水凝胶检测培养的大鼠中耳粘膜细胞的中耳粘膜再生

• 发表时间: 2014
• 作者: Naotaro Akiyama;Tomomi Yamamoto-Fukuda;Haruo Takahashi;Takehiko Koji;穐山直太郎
• 通讯作者: 穐山直太郎

Ultrastructure of regenerated middle ear mucosa after transplantation of cultured epithelial cells

培养上皮细胞移植后再生中耳粘膜的超微结构

• 发表时间: 2012
• 作者: Naotaro Akiyama;Tomomi Yamamoto-Fukuda;Haruo Takahashi;穐山直太郎;Naotaro Akiyama;Naotaro Akiyama
• 通讯作者: Naotaro Akiyama

Evaluation of in situ tissue engineering for mucosal regeneration of rat middle ear

原位组织工程对大鼠中耳粘膜再生的评价

• 发表时间: 2012
• 作者: Naotaro Akiyama;Tomomi Yamamoto-Fukuda;Haruo Takahashi;穐山直太郎;Naotaro Akiyama
• 通讯作者: Naotaro Akiyama

外耳道後壁軟組織再建術にM-meatoplasty を併用した手術症例の検討

M形口成形术联合外耳道后壁软组织重建手术病例研究

• 发表时间: 2012
• 作者: Naotaro Akiyama;Tomomi Yamamoto-Fukuda;Haruo Takahashi;穐山直太郎
• 通讯作者: 穐山直太郎