Differentiation induction from embryonic stem cells to functional thyroid tissues

胚胎干细胞向功能性甲状腺组织的分化诱导

• 批准号: 24791762
• 负责人: KANNO Masafumi
• 金额: $ 2.66万
• 依托单位: University of Fukui
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24791762/
• 关键词: ES細胞; 甲状腺; Ｌｒｈ－１; 胚性癌細胞; 甲状腺濾胞上皮細胞; 内胚葉; F9細胞; Lrh-1

Differentiation induction from embryonic stem cells to thyroid tissues, which we is our objective, was accomplished by Dr. Antonica and others from Belgium in 2012, leading to transplantation into the body of a mouse and thus to recovery of thyroid function (Nature 491(7422):66-71.2012).We inferred an important relation between this differentiation efficacy and the potency of embryonic stem cells to maintain the undifferentiated state. We analyzed it further to improve the differentiation induction efficacy of embryonic stem cells. Results demonstrated that the liver receptor homologue 1 (LRH1), which we specifically examined, was induced directly by Sox2 and Gabp. It played an important role in self renewal.

我们的目标是从胚胎干细胞诱导分化为甲状腺组织，由比利时的Antonica博士等人于2012年完成，移植到小鼠体内，从而恢复甲状腺功能（Nature 491（7422） ）：66-71.2012）。我们推断这种分化功效与胚胎干细胞维持未分化状态的能力之间存在重要关系。我们进一步对其进行分析，以提高胚胎干细胞的分化诱导功效。结果表明，我们专门检查的肝受体同源物 1 (LRH1) 是由 Sox2 和 Gabp 直接诱导的。它在自我更新方面发挥了重要作用。