The effect of antianxiety agent, saliva stimulant and gastric antisecretory activity on brux-like activity in rats

抗焦虑剂、唾液刺激剂和胃抗分泌活性对大鼠磨牙样活性的影响

基本信息

批准号：
18592249
负责人：
NAGATA Junko
金额：
$ 2.62万
依托单位：
Kagoshima University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

项目摘要

The aim of the present study was to investigate the relationship between the emotional stress and brux-like activity by comparison of the brain wave, activity of the masticatory muscles and expression frequency of the brux-like activity of rats those with emotional stress (emotional stress group) and without emotional stress (control group).First, it was essential to investigate the sleep stage such as arousal and REM sleep stage. However, it has never been established to investigate the sleep stage of the rats automatically by the brain wave. We examined the effectiveness of the existing chronological data analysis software by investigation of the human brain wave data that has been already analyzed by the scientific operator who can determine the sleep stage. In the result, it was certified that the chronological data analysis software was useful to determine the depth and stage of the sleep automatically with high degree of accuracy.Second, electrical pole screw was inserted to cranial bone of the 7-8 week-old Wister rats under general anesthesia and it was connected with copper line. Then, a needle electrode was inserted to the parietal region of the cranial bone through left side masseter muscle. These copper lines were connected with constantly embedded micro connecter and fixed to the parietal region. Brain wave, masseter muscle activity and electro-oculogram were developed using these devices.Additionally, a catheter was inserted into external jugular vein and fixed to dorsal region of jacket through skin. Then, frequency and its change of brux-like activity were investigated when emotional stress was applied.In the result, it was suggested that depth of sleep showed changes and the frequency of the brux-like activity increased when the emotional stress was applied. It was also suggested that premedication of antagonist drug to the emotional stress controled the increase of the brux-like activity.

本研究的目的是通过比较情绪应激大鼠的脑电波、咀嚼肌活动和磨牙样活动的表达频率，探讨情绪压力与磨牙样活动之间的关系。组）和没有情绪压力（对照组）。首先，有必要研究睡眠阶段，例如觉醒和快速眼动睡眠阶段。然而，通过脑电波自动研究大鼠睡眠阶段的方法尚未建立。我们通过调查人类脑电波数据来检查现有时间数据分析软件的有效性，这些数据已经由可以确定睡眠阶段的科学操作员分析过。结果证明，按时间顺序的数据分析软件可以自动高精度地确定睡眠深度和阶段。其次，将电杆螺钉插入 7-8 周龄 Wister 的颅骨中。大鼠全身麻醉，用铜线连接。然后，将针电极通过左侧咬肌插入颅骨的顶骨区域。这些铜线与不断嵌入的微型连接器连接并固定在顶叶区域。使用这些设备开发脑电波、咬肌活动和眼电图。此外，将导管插入颈外静脉并通过皮肤固定在夹克的背部区域。然后，研究了施加情绪压力时磨牙样活动的频率及其变化。结果表明，当施加情绪压力时，睡眠深度出现变化，磨牙样活动的频率增加。也有人提出，针对情绪应激预先施用拮抗药物可控制磨牙样活动的增加。