The relationship between dissolved organic matter cycling and bacterioplankton in freshwater environments

淡水环境中溶解有机物循环与浮游细菌的关系

• 批准号: 23710023
• 负责人: WATANABE Keiji
• 金额: $ 2.83万
• 依托单位: Center for Environmental Science in Saitama (2012)National Institute for Environmental Studies (2011)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23710023/
• 关键词: 微生物ループ; 物質循環; 溶存有機物; 浮遊細菌; 湖沼環境

We have succeeded to the isolation of many of dominant but unculturable freshwater bacterioplankton clusters/subclusters from the freshwater lakes and a river. These isolates were strongly dependent on the carboxylic acids as carbon source. Freshwater bacterioplankton subclusters (PnecC and PnecD) have shown the niche separation by the difference of main source of carboxylic acids in the field (photodegradation of humic substances or primary production). In addition, the production of chromophoric dissolved organic matter which has terrestrial humic-like fluorescence by part of freshwater clusters/subclusters was observed in the cultivation experiments. The contribution of freshwater bacterioplankton to carbon cycle has been partly cleared due to the cultivation-based approach using pure strains.

我们已经成功地隔离了许多淡水湖泊和河流中的许多主要但不可耕种的淡水型细菌群/子聚类。这些分离株强烈依赖于羧酸作为碳源。淡水细菌浮游生物子clusters（PNECC和PNECD）表明，通过田间中羧酸的主要来源（腐殖质物质的光降解或初级生产）的主要来源差异。此外，在培养实验中观察到了淡水簇/子观察者的一部分淡水簇/子观察者的发色生物溶解有机物的产生。由于使用纯菌株的基于培养的方法，淡水细菌对碳循环的贡献已部分清除。