Mechanism of oriented-axon outgrowth in developmentalbrain

发育脑定向轴突生长的机制

• 批准号: 23700448
• 负责人: TORIYAMA Michinori
• 金额: $ 2.83万
• 依托单位: Nara Institute of Science and Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23700448/
• 关键词: 脳・神経; 発生・分化; 神経科学; 細胞極性; 軸索ガイダンス; 軸索伸長; shootin1; netrin-1; 軸索; 成長円錐; Shootin1; Netrin-1; クラッチ分子

Here we show that shootin1 is located at a critical interface, transducing a chemical signal into traction forces for axon outgrowth. We found that a netrin-1 positively regulates Pak1-mediated shootin1 phosphorylation. This phosphorylation enhanced the interaction between shootin1 and F-actin retrograde flow, thereby promoting F-actin-substrate coupling, force generation, and axon outgrowth. These results suggest that dynamic actin-substrate coupling can transduce chemical signals into mechanical forces to control cellular motility and provide a molecular-level description of how this transduction may occur.

在这里，我们表明shotin1位于临界界面，将化学信号转换为轴突生长的牵引力。我们发现Netrin-1阳性调节PAK1介导的Shotin1磷酸化。这种磷酸化增强了Shotin1与F-肌动蛋白逆行流之间的相互作用，从而促进了F-肌动蛋白 - 基底耦合，力产生和轴突的生长。这些结果表明，动态肌动蛋白 - 基底耦合可以将化学信号转移到机械力中，以控制细胞运动，并提供有关该转导的分子级别描述。

项目成果

Rab33a Mediates Anterograde Vesicular Transport for Membrane Exocytosis and Axon Outgrowth

• DOI: 10.1523/jneurosci.0989-12.2012
• 发表时间: 2012-09-12
• 期刊: JOURNAL OF NEUROSCIENCE
• 影响因子: 5.3
• 作者: Nakazawa, Hitomi;Sada, Tadayuki;Inagaki, Naoyuki
• 通讯作者: Inagaki, Naoyuki

Conversion of a Signal into Forces for Axon Outgrowth through Pak1-Mediated Shootin1 Phosphorylation

• DOI: 10.1016/j.cub.2013.02.017
• 发表时间: 2013-03-18
• 期刊: CURRENT BIOLOGY
• 影响因子: 9.2
• 作者: Toriyama, Michinori;Kozawa, Satoshi;Inagaki, Naoyuki
• 通讯作者: Inagaki, Naoyuki

Netrin-1 Regulates Pak1-mediated Phosphorylation of Shootin1: Modulation of Clutch Activity of Shootin1 for Axon Outgrowth.

Netrin-1 调节 Pak1 介导的 Shootin1 磷酸化：调节 Shootin1 的离合器活性以促进轴突生长。

• 发表时间: 2011
• 作者: Michinori Toriyama;Naoyuki Inagaki
• 通讯作者: Naoyuki Inagaki

Netrin-1 Regulates Pak1-mediated Phosphorylation of Shootin1: Modulation of Clutch Activity of Shootin1 for Axon Outgrowth

Netrin-1 调节 Pak1 介导的 Shootin1 磷酸化：调节 Shootin1 的离合器活性以促进轴突生长

• 发表时间: 2011
• 作者: Toriyama M;Inagaki;N.
• 通讯作者: N.

A diffusion-base neurite length-sensing mechanism involved in neuronal polarization

参与神经元极化的基于扩散的神经突长度传感机制

• 发表时间: 2011
• 作者: Michinori Toriyama;Yuichi Sakumura;Tadayuki Shimada;Shin Ishii;Naoyuki Inagaki
• 通讯作者: Naoyuki Inagaki