Identification and functional analysis of novel bioactive peptides that regulate energy metabolism and food intake

调节能量代谢和食物摄入的新型生物活性肽的鉴定和功能分析

• 批准号: 23791066
• 负责人: YOSHIDA Morikatsu
• 金额: $ 2.66万
• 依托单位: National Cardiovascular Center Research Institute
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23791066/
• 关键词: 生理活性ペプチド; オーファン受容体; 摂食・エネルギー代謝調節; 神経ペプチド; ペプチドホルモン; オーファンGPCR; 摂食行動; エネルギー代謝調節; エネルギー代謝

We performed a search for newly bioactive peptides acting on orphan receptors expressed in the hypothalamus which controls the energy metabolism and feeding behavior. For screening candidate ligands of these receptors, we developed novel activity detection system which is determined by microscopic morphological changes of cells (CellKey assay). Candidate ligands in tissue extracts were screened against cell lines expressing target orphan receptors by using this system. In cells expressing a certain orphan receptor, we detected the significant increase of impedance, which was the target receptor specific. We are now proceeding to purify the active peptide from this extract.

我们寻找作用于下丘脑中表达的孤儿受体的新生物活性肽，该孤儿受体控制能量代谢和进食行为。为了筛选这些受体的候选配体，我们开发了新型活性检测系统，该系统通过细胞的微观形态变化来确定（CellKey 测定）。使用该系统针对表达目标孤儿受体的细胞系筛选组织提取物中的候选配体。在表达某种孤儿受体的细胞中，我们检测到阻抗显着增加，这是目标受体特异性的。我们现在正着手从该提取物中纯化活性肽。

项目成果

Isolation of the bioactive peptides CCHamide-1 and CCHamide-2 from Drosophila and their putative role in appetite regulation as ligands for G protein-coupled receptors.

• DOI: 10.3389/fendo.2012.00177
• 发表时间: 2012
• 期刊: Frontiers in endocrinology
• 影响因子: 5.2
• 作者: Ida T;Takahashi T;Tominaga H;Sato T;Sano H;Kume K;Ozaki M;Hiraguchi T;Shiotani H;Terajima S;Nakamura Y;Mori K;Yoshida M;Kato J;Murakami N;Miyazato M;Kangawa K;Kojima M
• 通讯作者: Kojima M

(招聘講義) 抗体の精製・抗体アフィニティの基礎から応用まで,宮崎大学IR推進機構テクニカルセミナー(2012.8.28～30宮崎)

（特邀讲座）从抗体纯化和抗体亲和力的基础知识到应用，宫崎大学IR推进组织技术研讨会（2012.8.28-30宫崎）

Methods in enzymology

• DOI: 10.1016/s0076-6879(88)65019-1
• 发表时间: 1988-01-01
• 期刊: Methods Enzymol
• 作者: Leppla, SH.

Orphan GPCRs and methods for identifying their ligands. Methods Enzymol.

孤儿 GPCR 及其配体的鉴定方法。

• 发表时间: 2012
• 作者: oshida;M.;Miyazato;M.;Kangawa;K.
• 通讯作者: K.