Dermal wound aging by molecular biological detections of growth factors

通过生长因子的分子生物学检测真皮伤口老化

• 批准号: 18590642
• 负责人: AOKI Yasuhiro
• 金额: $ 1.73万
• 依托单位: Iwate Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18590642/
• 关键词: skin; brain; bone; cytokine; growth factor; real-time quantitative PCR; in situ hybridization; beads suspension array; 損傷; beads suspension array; quantitave PCR; エイジング; 創傷治癒; 定量的PCR

Dermal wound age provides forensic pathologists with valuable information for the reconstruction of crime scenes and determination of the cause of death. In this study, cytokine expressions in murine skins were analyzed using immunohistochemical methods, real-time quantitative PCR, and in situ hybridization techniques. Wound age estimation for human dermal wounds was also performed based on quantification of 17 cytokines. The potential parameters would be IL 5, IL 10, IL 12 p 70, IL 13, IL 17, GM-CSF, IFN γ, TNF α in the early phase of dermal wound healing, IL 6, MCP 1 in the middle phase, and IL 1 β, IL 2, IL 4, IL 8, G-CSF, and MIP 1β in the middle and late phases. In addition, an outsourced examination of IL 1 β, IL 4, IL 6, IL 8, IL 10, G-CSF, GM-CSF, IFN γ, MCP1, TNFα, which could be available to any forensic institute, was performed for confirmative purposes. Many factors have been proposed as markers for dermal wound age estimation, but the set of cytokines selected for the outsourced examination in the present study wound be useful in daily forensic practice.

真皮年龄伤口为法医病理学家重建犯罪现场和确定死因提供了有价值的信息。在这项研究中，使用免疫组织化学方法、实时定量PCR和原位杂交技术分析了小鼠皮肤中的细胞因子表达。还根据 17 种细胞因子的定量进行了人类皮肤伤口的伤口年龄估计，潜在参数为 IL 5、IL 10、IL 12 p 70、IL 13、IL 17、真皮伤口愈合早期为 GM-CSF、IFN γ、TNF α，中期为 IL 6、MCP 1，后期为 IL 1 β、IL 2、IL 4、IL 8、G-CSF 和 MIP 1β。中后期另外还外包检测IL 1 β、IL 4、IL 6、IL 8、IL 10、G-CSF、GM-CSF、IFN γ、 MCP1、TNFα 可用于任何法医机构，用于确认目的，已提出许多因素作为真皮伤口年龄估计的标记，但在本研究伤口中选择用于外包检查的细胞因子组是有用的。日常法医实践。

项目成果

An autopsy case related to a terrorist attack using a ball-bearing bomb.

与使用滚珠炸弹的恐怖袭击有关的尸检案件。

• 作者: Masataka Takamiya. Hitoshi Biwasaka; Hisae Niitsu; Kiyoshi Saigusa; Yasuhiro Aoki
• 通讯作者: Yasuhiro Aoki

A case of carbamazepine overdose with focal myocarditis.

卡马西平过量致局灶性心肌炎一例。

• 发表时间: 2006
• 作者: Takamiya M; Aoki Y; Niitsu H; Saiguwa K
• 通讯作者: Saiguwa K

A case of Takayasu's arteritis complicated by right atrium perforation and injuries of the right common iliac artery and vein caused by cannulation for percutaneous cardiopulmonary support.

经皮心肺支持插管致高安动脉炎并发右心房穿孔及右髂总动静脉损伤1例。

• 作者: Masataka Takamiya; Sashiko Fujita; Hisae Niitsu; Yasuhiro Aoki; Hiroyuki Kanno; Takashi Sawai
• 通讯作者: Takashi Sawai

骨折治癒過程におけるtPAおよびuPA遺伝子の発現動態

骨折愈合过程中tPA和uPA基因的表达动态

• 发表时间: 2006
• 作者: 高宮正隆; 熊谷礼子; 中屋敷徳; 青木康博.
• 通讯作者: 青木康博.

Simultaneous detection of eight cytokines in human dermal wounds with a multiplex bead-based immunoassay for wound age estimation

使用基于多重微珠的免疫测定法同时检测人类真皮伤口中的八种细胞因子，以估计伤口年龄

• DOI: 10.1007/s00414-007-0183-5
• 发表时间: 2008-03-01
• 期刊: International Journal of Legal Medicine
• 影响因子: 2.1
• 作者: M. Takamiya;S. Fujita;K. Saigusa;Y. Aoki
• 通讯作者: Y. Aoki