Studies on mRNA Expressions of Cytokines during Wound Healing for Determination of Wound Age.

研究伤口愈合过程中细胞因子 mRNA 表达以确定伤口年龄。

基本信息

批准号：
12670402
负责人：
AOKI Yasuhiro
金额：
$ 1.73万
依托单位：
Iwate Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12670402/
关键词：
injury wound aging wound healing bFGF VEGF fibronectin quantitative PCR in situ hybridization サイトカイン遺伝子発現 fibronectin 免疫組織化学損傷創傷治癒法医学

项目摘要

An animal experimental study was carried out to investigate availability of mRNA expressions of cytekines as markers for wound aging. A 2 cm full thickness incision was made on the dorsal skin of male DDY mice of 5 to 6 weeks old, and they were killed 0.5, 1, 3, 8, 24, 72, 144, or 240 hours after the incision. The expression of basic fibroblast growth factor (bFGF) and vascular endothelial growth factor (VEGF) were examined immunohistochemically using specific antibodies. For wound age determination, bFGF, which was detected in the early 0.5- to 1-hour phases and the late 24- to 144-hour phases, seemed to be superior to VEGF because of its earlier expression and because of its more persistent expression in dermal fibroblasts with increasing postomortem interval. Therefore, expressions of bFGF mRNA during wound healing were subsequently analyzed using quantitative PCR and in situ hybridization techniques. Beside the dermal wound, mRNA dynamics of dermal, cerebral, hepatic and renal ones were also examined. bFGF mRNA expressions in the injured skin peaked at 1 hour, and they were detected in epidermal cells, fibroblasts, endothelial cells and neutrophils. Those of the injured cerebrum increased from 1 hour and peaked at 48 hours. The astrocytes were main source of vFGF mRNA supply in the injured brain. In hepatic and renal injuries, however, bFGF mRNA expressions increased slightly at 24 hours in endothelial cells, in neutrophils of the liver, and in glomeruli of the kidney. These results suggested that the mRNA dynamics of skin and brain derived from ectoderm were quite different from those of liver and kidney. In other words, time-dependent expressions of bFGF mRNA could be useful wound aging marker for the organs of ectodermal origin.

进行了一项动物实验研究，以研究细胞因子 mRNA 表达作为伤口老化标志物的可用性。在5至6周龄的雄性DDY小鼠的背部皮肤上切开2cm全层切口，并在切口后0.5、1、3、8、24、72、144或240小时处死它们。使用特异性抗体进行免疫组织化学检查碱性成纤维细胞生长因子（bFGF）和血管内皮生长因子（VEGF）的表达。对于伤口年龄的测定，在早期 0.5 至 1 小时阶段和晚期 24 至 144 小时阶段检测到的 bFGF 似乎优于 VEGF，因为它的表达更早，并且在伤口中更持久地表达。真皮成纤维细胞随着死后间隔的增加而增加。因此，随后使用定量PCR和原位杂交技术分析了伤口愈合过程中bFGF mRNA的表达。除了真皮伤口外，还检查了真皮、大脑、肝脏和肾脏伤口的 mRNA 动态。受伤皮肤中bFGF mRNA表达在1小时达到峰值，并在表皮细胞、成纤维细胞、内皮细胞和中性粒细胞中检测到。受伤的大脑从1小时开始增加，并在48小时达到顶峰。星形胶质细胞是受伤大脑中 vFGF mRNA 供应的主要来源。然而，在肝和肾损伤中，24小时内皮细胞、肝脏中性粒细胞和肾脏肾小球中的bFGF mRNA表达略有增加。这些结果表明，源自外胚层的皮肤和大脑的 mRNA 动态与肝脏和肾脏的 mRNA 动态有很大不同。换句话说，bFGF mRNA 的时间依赖性表达可能是外胚层起源器官的有用的伤口老化标记。