Development of endosome targeted peptide inhibitors against envelopedviruses via endosomal pathway.

通过内体途径开发针对包膜病毒的内体靶向肽抑制剂。

• 批准号: 23790509
• 负责人: UJIKE Makoto
• 金额: $ 2.83万
• 依托单位: Nippon Veterinary and Life Science University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23790509/
• 关键词: コロナウイルス; S 蛋白質; 膜融合; 抗ウイルス薬; HRP; 細胞侵入; Heptad-repeat; コレステロール付加ペプチド; Heptad-Repeat

Short peptides (HRP), derived from the heptad repeat region of spike proteins on enveloped viruses using cell surface entry pathway, have been shown to block fusion activity, resulting in viral infection inhibition. However, HRPs have a limited ability to transfer into endosomes, therefore they show low or insignificant antiviral activity for enveloped viruses using the endosomal entry pathway. In this study, we have designed cholesterol conjugated HRPs(HRP-chol) which can be targeted to endosomes and evaluated their antviral activities of cell surface or endosomal entry pathways of several coronaviruses(CoV). Our results indicated that HRP-chol showed potent antiviral activities against not only surface but endosomal entry pathways of several CoVs. These results suggested that HRP-chol antiviral strategy may be applied to broad-ranged enveloped viruses using the endosomal pathway.

短肽 (HRP) 源自有包膜病毒上刺突蛋白的七肽重复区域，利用细胞表面进入途径，已被证明可以阻断融合活性，从而抑制病毒感染。然而，HRP 转移到内体的能力有限，因此它们对使用内体进入途径的包膜病毒表现出较低或不显着的抗病毒活性。在本研究中，我们设计了可靶向内体的胆固醇缀合HRP（HRP-chol），并评估了其对几种冠状病毒（CoV）的细胞表面或内体进入途径的抗病毒活性。我们的结果表明，HRP-chol 不仅对几种 CoV 的表面进入途径而且对内体进入途径均表现出有效的抗病毒活性。这些结果表明，HRP-chol 抗病毒策略可通过内体途径应用于广泛的包膜病毒。

项目成果

野外株より細胞培養インフルエンザワクチンの種候補株を選定する基準の検討

从野外毒株中选择细胞培养流感疫苗候选毒株的标准的审查

• 发表时间: 2012
• 作者: Yoshida;A.;Sriwilaijaroen N;浅沼秀樹
• 通讯作者: 浅沼秀樹

Hemagglutination mediated by the spike protein of cell-adapted bovine torovirus.

• DOI: 10.1007/s00705-013-1636-4
• 发表时间: 2013-07
• 期刊: Archives of virology
• 影响因子: 2.7
• 作者: Shimabukuro K;Ujike M;Ito T;Tsunemitsu H;Oshitani H;Taguchi F
• 通讯作者: Taguchi F

Mutation in the cytoplasmic retrieval signal of porcine epidemic diarrhea virus spike (S) protein is responsible for enhanced fusion activity.

• DOI: 10.1016/j.virusres.2011.07.019
• 发表时间: 2011-11
• 期刊: Virus research
• 影响因子: 5
• 作者: Shirato K;Maejima M;Matsuyama S;Ujike M;Miyazaki A;Takeyama N;Ikeda H;Taguchi F
• 通讯作者: Taguchi F

SARS-CoVの粒子形成におけるERretrieval signalの役割について

关于 ERretrieval 信号在 SARS-CoV 颗粒形成中的作用

• 发表时间: 2012
• 作者: 氏家誠;白戸憲也;松山州徳;田口文広
• 通讯作者: 田口文広

Monitoring and characterization of oseltamivir-resistant pandemic (H1N1) 2009 virus, Japan, 2009-2010.

• DOI: 10.3201/eid1703.101188
• 发表时间: 2011-03
• 期刊: Emerging infectious diseases
• 影响因子: 11.8
• 作者: Ujike M;Ejima M;Anraku A;Shimabukuro K;Obuchi M;Kishida N;Hong X;Takashita E;Fujisaki S;Yamashita K;Horikawa H;Kato Y;Oguchi A;Fujita N;Tashiro M;Odagiri T;Influenza Virus Surveillance Group of Japan
• 通讯作者: Influenza Virus Surveillance Group of Japan