The role of excitatory action of GABA in the estrous cycle of mouse

GABA兴奋作用在小鼠动情周期中的作用

• 批准号: 23790283
• 负责人: WATANABE Miho
• 金额: $ 2.83万
• 依托单位: Hamamatsu University School of Medicine (2012-2013)Gunma University (2011)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23790283/
• 关键词: GnRHニューロン; GABA; KCC2; 視床下部; 神経内分泌学; NKCC1; 性周期; 生殖生理; 神経科学

Gonadotropin-releasing hormone (GnRH) neurons form the final common pathway for the central regulation of reproduction. Previously, we reported that GABA, the main inhibitory neurotransmitter in the adult brain, exerts an excitatory action in adult GnRH neurons. To examine the functional role of the excitatory action of GABA in GnRH neurons in vivo, we generated transgenic mice with conditional overexpression of KCC2 or knockout of NKCC1 restricted in GnRH neurons in a reversible fashion using tetracycline controlled gene expression system. Female mice, which expressed KCC2 in GnRH neurons, showed no pregnancy and abnormal estrous cyclicity and abnormal ovary containing many small follicles. These results suggest that the excitatory action of GABA on GnRH neurons has an important role in the female reproduction.

促性腺激素释放激素（GNRH）神经元构成了繁殖中心调节的最终公共途径。以前，我们报道了成年大脑中主要抑制性神经递质GABA在成年GNRH神经元中发挥兴奋性作用。为了检查GABA在体内GNRH神经元中的兴奋作用的功能作用，我们使用TertracyClaceLancline Controled Gene表达系统以可逆性的方式产生了具有KCC2的条件过表达KCC2或NKCC1限制的NKCC1的转基因小鼠。在GNRH神经元中表达KCC2的雌性小鼠没有妊娠和异常发情环节性和含有许多小卵泡的卵巢异常。这些结果表明，GABA对GNRH神经元的兴奋作用在女性繁殖中具有重要作用。

项目成果

GABA regulates the multidirectional tangential migration of GABAergic interneurons in living neonatal mice.

• DOI: 10.1371/journal.pone.0027048
• 发表时间: 2011
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Inada H;Watanabe M;Uchida T;Ishibashi H;Wake H;Nemoto T;Yanagawa Y;Fukuda A;Nabekura J
• 通讯作者: Nabekura J

GnRHニューロンにおける興奮性GABA作用の役割

兴奋性 GABA 作用在 GnRH 神经元中的作用

• 发表时间: 2011
• 作者: 渡部美穂;加藤昌克;佐久間康夫;鍋倉淳一
• 通讯作者: 鍋倉淳一

Inter-regional Contribution of Enhanced Activity of the Primary Somatosensory Cortex to the Anterior Cingulate Cortex Accelerates Chronic Pain Behavior

• DOI: 10.1523/jneurosci.0946-11.2011
• 发表时间: 2011-05-25
• 期刊: JOURNAL OF NEUROSCIENCE
• 影响因子: 5.3
• 作者: Eto, Kei;Wake, Hiroaki;Nabekura, Junichi
• 通讯作者: Nabekura, Junichi

Inter-regional contribution of enhanced activity of the primary somatosensory cortex to the anterior cingulated cortex accelerates chronic pain behavior.

初级体感皮层对前扣带皮层活性增强的区域间贡献加速了慢性疼痛行为。

• 发表时间: 2011
• 期刊: Journal of Neuroscience
• 影响因子: 5.3
• 作者: Eto K;Wake H;Watanabe M;Ishibashi H;Noda M;Yanagawa Y;Nabekura J
• 通讯作者: Nabekura J

GnRH ニューロンで KCC2 を過剰発現させたマウスでは雌の生殖機能に変化がみられる

GnRH 神经元中过度表达 KCC2 的小鼠显示出雌性生殖功能的变化。

• 作者: 渡部美穂;鍋倉淳一
• 通讯作者: 鍋倉淳一