Neutron Crystallographic study of hydration structures of DNA and its fluctuation
DNA水合结构及其波动的中子晶体学研究
基本信息
- 批准号:23770176
- 负责人:
- 金额:$ 2.75万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Young Scientists (B)
- 财政年份:2011
- 资助国家:日本
- 起止时间:2011 至 2013
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
For the purpose of revealing structure of DNA hydration including structural fluctuation, neutron and X-ray crystallographic studies were carried out. (1) Deuterated Z-DNA d(CGCGCG) could be produced. (2) Large single crystals of Z-DNA d(CGCGCG) were obtained from H2O solution, and TOF neutron experiment was performed using pulsed neutron source. Neutron diffraction spots were detected up to 1.6 -1.8 Angstrom. Protocol for refinements was tested using neutron data sets of protein crystals. X-ray crystallographic analyses were also carried out complementarily, as the result, Z-DNA structures at high and low concentrations of metal salts were determined. Each of these structures had specific features, suggesting that these crystals would be dominant candidates for neutron crystallographic studies.
为了揭示DNA水合的结构,包括结构波动,中子和X射线晶体学研究。 (1)可以产生氘代Z-DNA D(CGCGCG)。 (2)从H2O溶液中获得Z-DNA D(CGCGCG)的大型单晶,并使用脉冲中子源进行TOF中子实验。检测到中子衍射斑点高达1.6 -1.8 Angstrom。使用蛋白质晶体的中子数据集测试了改进方案。还对X射线晶体学分析进行了互补的补充,因为结果确定了高浓度的金属盐和低浓度的Z-DNA结构。这些结构中的每一个都有特定的特征,表明这些晶体将是中子晶体学研究的主要候选者。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Direct interactions between Z-DNA and alkaline earth cations, discovered in the presence of high concentrations of MgCl2 and CaCl2
- DOI:10.1016/j.jinorgbio.2013.03.004
- 发表时间:2013-07-01
- 期刊:
- 影响因子:3.9
- 作者:Chatake, Toshiyuki;Sunami, Tomoko
- 通讯作者:Sunami, Tomoko
高塩状態で誘導されるDNA会合の研究
高盐条件诱导DNA缔合的研究
- DOI:
- 发表时间:2012
- 期刊:
- 影响因子:0
- 作者:樋口芳樹; 茶竹俊行; 横山武司; 山田太郎 (代表発表者: 樋口芳樹);白崎 善隆;茶竹俊行
- 通讯作者:茶竹俊行
Time of Flight Neutron Crystallographic analysis of Human Carbonmonoxyhemoglobin
人碳单氧血红蛋白的飞行时间中子晶体分析
- DOI:
- 发表时间:2011
- 期刊:
- 影响因子:0
- 作者:T. Chatake;N. Shibayama;S.Y. Park;K. Tomoyori;T. Hosoya;T. Ohara;K. Kusaka;K. Kurihara;I. Tanaka;N. Niimura;Y. Morimoto
- 通讯作者:Y. Morimoto
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CHATAKE Toshiyuki其他文献
CHATAKE Toshiyuki的其他文献
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{{ truncateString('CHATAKE Toshiyuki', 18)}}的其他基金
X-ray crystallographic analysis of DNA using temperature-control crystallization technique
使用温控结晶技术对 DNA 进行 X 射线晶体学分析
- 批准号:
18790030 - 财政年份:2006
- 资助金额:
$ 2.75万 - 项目类别:
Grant-in-Aid for Young Scientists (B)
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