Determination of absorption spectra of reaction intermediates to fully understand the reaction mechanism of cytochrome oxidase

测定反应中间体的吸收光谱，全面了解细胞色素氧化酶的反应机理

• 批准号: 23770152
• 负责人: YANAGISAWA SACHIKO
• 金额: $ 3万
• 依托单位: University of Hyogo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23770152/
• 关键词: 金属タンパク質; 生体エネルギー変換; 振動分光法; 酸素活性化; 振動分光; 吸収スペクトル

We isolate electrons from food by digestion and transfer them to dioxygen, generating water molecules. This oxygen reduction reaction is catalyzed by cytochrome oxidase and coupled with proton pumping reaction. The coupling mechanism of these two reactions is still unknown and is investigated by various structural chemical techniques. To understand the mechanisms we need to link all those results in logical manner with a clear benchmark. Absorption spectra of those intermediates can be benchmarks and it is now ready to measure those of intermediates.

我们通过消化从食物中分离出电子并将其转移到双氧中，产生水分子。这种氧还原反应由细胞色素氧化酶催化并与质子泵反应相结合。这两个反应的耦合机制仍然未知，并通过各种结构化学技术进行研究。为了理解这些机制，我们需要以逻辑方式将所有这些结果与明确的基准联系起来。这些中间体的吸收光谱可以作为基准，现在可以测量中间体的吸收光谱。

项目成果

The influence of active site loop mutations on the thermal stability of azurin from Pseudomonas aeruginosa

• DOI: 10.1016/j.abb.2012.03.007
• 发表时间: 2012-05-01
• 期刊: ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
• 影响因子: 3.9
• 作者: Guzzi, Rita;Sportelli, Luigi;Dennison, Christopher
• 通讯作者: Dennison, Christopher

Interaction of dimeric horse cytochrome c with cyanide ion

• DOI: 10.1007/s00775-013-0982-8
• 发表时间: 2013-02
• 期刊: JBIC Journal of Biological Inorganic Chemistry
• 作者: A. Nugraheni;S. Nagao;S. Yanagisawa;T. Ogura;S. Hirota

へムをへム垂線のまわりに900回転した時の共鳴ラマンスペクトル変化 : へムオキシゲネース

血红素绕血红素垂直线旋转 900 次时共振拉曼光谱变化：血红素加氧酶

• 发表时间: 2012
• 作者: Yamamoto;H.;Kida;Y.;and Sakaguchi;M;Sachiko Yanagisawa,Hiroshi Fujii,Saburo Neya,Takashi Ogura and Teizo Kitagawa,"Effects of Heme Rotation around the Heme Normal by 90 0 on Resonance Raman Spectra of Heme Proteins; Observation for Heme Oxygenase
• 通讯作者: Sachiko Yanagisawa,Hiroshi Fujii,Saburo Neya,Takashi Ogura and Teizo Kitagawa,"Effects of Heme Rotation around the Heme Normal by 90 0 on Resonance Raman Spectra of Heme Proteins; Observation for Heme Oxygenase

Dioxygenation reaction of indoleamine 2,3-dioxygenase as studied by resonance Raman spectroscopy

共振拉曼光谱研究吲哚胺2,3-双加氧酶的双加氧反应

• 发表时间: 2012
• 作者: 田邊賢司;森川由章;逸見祐次;池田なるみ;大江夏子;目崎久美;竹居孝二;Sachiko Yanagisawa,Masayuki Hara,Hiroshi Sugimoto,Yoshitsugu Shiro and Takashi Ogura
• 通讯作者: Sachiko Yanagisawa,Masayuki Hara,Hiroshi Sugimoto,Yoshitsugu Shiro and Takashi Ogura

Effects of Heme Rotation around the Heme Normal by 90 0 on Resonance Raman Spectra of Heme Proteins; Observation for Heme Oxygenase

血红素围绕血红素法线旋转 90 0 对血红素蛋白共振拉曼光谱的影响；

• 发表时间: 2012
• 作者: 逸見祐次;田邊賢司;竹居孝二;Sachiko Yanagisawa,Hiroshi Fujii,Saburo Neya,Takashi Ogura,and Teizo Kitagawa
• 通讯作者: Sachiko Yanagisawa,Hiroshi Fujii,Saburo Neya,Takashi Ogura,and Teizo Kitagawa