The analysis of expression state of HOX family genes and their effects for cancer-associated in oral cancer

口腔癌HOX家族基因表达状态及其癌相关作用分析

• 批准号: 23792317
• 负责人: KOUZU Yukinao
• 金额: $ 2.66万
• 依托单位: Chiba University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23792317/
• 关键词: HOX family; HOXA1; 口腔癌; マイクロアレイ; siRNA; ホメオティック遺伝子; HOX遺伝子family

The purpose of this study was to examine the potential relationship of oral squamous cell carcinoma (OSCC) and HOX family which is transcription factors. We evaluated the status of HOX genes mRNA expression in OSCC cell lines by real-time PCR. The expression of HOXA1 and oral cancer related genes were particularly increased in all OSCC-derived cell lines. Similar to cell lines, increased expression was observed at mRNA and protein levels in clinical specimens. In addition, suppression of HOXA1 expression with siRNA significantly inhibited cell proliferation compared with the control cells. We suggested that HOXA1 and oral cancer related gene are closely related to OSCC and HOXA1 may play an important role in tumor progression.

这项研究的目的是检查口服鳞状细胞癌（OSCC）和HOX家族的潜在关系，即转录因子。我们通过实时PCR评估了OSCC细胞系中HOX基因mRNA表达的状态。在OSCC衍生的所有细胞系中，HOXA1和口腔癌相关基因的表达尤其升高。与细胞系相似，在临床标本中观察到在mRNA和蛋白水平上观察到的表达增加。另外，与对照细胞相比，用siRNA抑制HOXA1表达可显着抑制细胞的增殖。我们建议HOXA1和与口腔癌相关的基因与OSCC密切相关，Hoxa1可能在肿瘤进展中起重要作用。