Elucidation of the role of the ectopic aquaporin 1 in the onset of hydrocephalus of the kaolin-induced hydrocephalus model in rat

阐明异位水通道蛋白 1 在高岭土诱导的大鼠脑积水模型脑积水发病中的作用

• 批准号: 23791613
• 负责人: FUJITA Masataka
• 金额: $ 2.66万
• 依托单位: Nagoya City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23791613/
• 关键词: 実験脳外科学; RNA干渉; アクアポリン; 水頭症; RNAi; 水チャンネル; 中枢神経; ラット

We examined how ectopic AQP1, which increased at the development of hydrocephalus, influenced the hydrocephalus formation using the kaolin-induced hydrocephalus model in rat by RNAi knockdown. As the preparation, production of rabbit anti-AQP1 serum suitable for a Western blot was successful and the arrangement of siRNA suitable for knockdown was found out. However, the suitable method of the injection for in vivo RNAi could not establish within this period, so it remains as a future subject.

我们研究了在脑积水发展时增加的异位AQP1如何使用高岭土诱导的脑积水模型在RNAi敲低中影响脑积水形成。随着准备工作，适合蛋白质印迹的兔抗AQP1血清的产生成功，并发现了适合敲低的siRNA排列。但是，在此期间无法建立体内RNAi注射的合适方法，因此它仍然是未来的主题。