Mechanism of Genome Damage Response Induced by Ultrasoun

超声诱导基因组损伤反应机制

• 批准号: 23650304
• 负责人: KONDO Takashi
• 金额: $ 2.33万
• 依托单位: University of Toyama
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23650304/
• 关键词: 超音波・DNA損傷; 二本鎖切断; リン酸化ヒストンH2AX; キャビテーション; フリーラジカル; DNA-PK; DNA損傷; ATM; ATR; DNA二本鎖切断

A likely candidate process has been suggested to involve sonochemical activity, where reactive oxygen species mediate the generation of DNAsingle-strand breaks. Here however, we provide compelling new evidence that strongly supports a purely mechanical mechanism. Moreover, by a combination of specific assays （neutral comet tail and staining for γH2AX foci formation) we demonstrate for the first time that US exposure at even moderate intensities exhibits DNAdouble-strand breaks, through its facility to generate DNA damage across multiple cancer lines. Notably, colocalization assays highlight that ionizing radiation and ultrasound have distinctly different signatures to their respective γH2AX foci formation patterns, likely reflecting the different stress distributions that initiated damage formation.

一个可能的候选过程被认为涉及声化学活性，其中活性氧介导 DNA 单链断裂的产生，然而，我们在这里提供了令人信服的新证据，有力地支持了纯粹的中性彗尾和染色的 γH2AX 焦点形成。 ）我们首次证明，即使是中等强度的美国暴露也会出现 DNA 双链断裂，通过其设施可以在多种癌症细胞系中产生 DNA 损伤。共定位分析强调，电离辐射和超声对其各自的 γH2AX 病灶形成模式具有明显不同的特征，可能反映了引发损伤形成的不同应力分布。

项目成果

Differential cytotoxicity and sonosensitization by sanazole: Effect of cell type and acoustic parameters

桑唑的差异细胞毒性和声敏化：细胞类型和声学参数的影响

• DOI: 10.1007/s10396-010-0295-2
• 发表时间: 2011
• 期刊: J. Med. Ultrasonics
• 作者: Hassan MA;Furusawa Y;Zhao Q.L.;Takasaki I;Ferl LB Jr.;Tachibana K;Minemura M.;Sugiyama T;and Kondo T
• 通讯作者: and Kondo T

Enhanced gene transfection using calcium phosphate co-precipitates and low-intensity pulsed ultrasound

• DOI: 10.1016/j.ejps.2012.08.007
• 发表时间: 2012-11-20
• 期刊: EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
• 影响因子: 4.6
• 作者: Hassan, Mariame A.;Ahmed, Iman S.;Kondo, Takashi
• 通讯作者: Kondo, Takashi

Enhancement of ultrasoundor hyperthermia-induced cancer cell killing by antibacterial agents

抗菌剂增强超声或热疗诱导的癌细胞杀伤作用

• 发表时间: 2012
• 作者: Feril LB;Tachibana K;Kondo T;Ogawa R;Cui ZG
• 通讯作者: Cui ZG

Mariame MA, Furusawa Y, Minemura M, and Kondo T

Mariame MA、Furusawa Y、Minemura M 和 Kondo T

• 发表时间: 2011
• 作者: Mariame MA;Furusawa Y;Minemura M;and Kondo T
• 通讯作者: and Kondo T

Gene regulation and sonotransfection by loe-intensity ultrasound resulting in malinant melanoma inhibition

通过洛伊强度超声进行基因调控和声转染，抑制恶性黑色素瘤

• 发表时间: 2011
• 作者: Feril LB Jr;Tachibana K;Yamaguchi K;Ikeda-Dantsuji Y;Kondo T;Tabuchi Y;Furusawa Y;and Takasaki I
• 通讯作者: and Takasaki I