Development of tissue engineered oral mucosa maintaining higher regenerative potential -application of oral mucosa progenitor/stem cells-

开发保持较高再生潜力的组织工程口腔粘膜-口腔粘膜祖细胞/干细胞的应用-

• 批准号: 23592982
• 负责人: YOSHIZAWA Michiko
• 金额: $ 3.33万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23592982/
• 关键词: 移植・再生医療; 口腔顎顔面再建外科学; 口腔粘膜

We have investigated the application of ex vivo-produced oral mucosa equivalent (EVPOME) and its ability of oral mucosa regeneration. However, cultivated epithelial cells using the present methods has heterogeneity, consequently, there is a risk that EVPOME possess poor cell growth activity and capability of oral mucosa regeneration. The objective of the present study was to investigate the capability of EVPOME fabricated with small-sized cell population in which oral mucosal progenitor/stem-cell-enriched subpopulation present and the change after EVPOME grafting subcutaneously in mice histologically. More Ki-67 positive cells were observed in the epithelial cells of EPOME fabricated by small-sized cell population. Epithelial elongation of EVPOME fabricated by small-sized cell population occurs faster than that of EVPOME using present methods. These findings suggest that EVPOME with small-sized cell population has high activity and capability of oral mucosal regeneration.

我们研究了离体产生的口腔粘膜等效物（EVPOME）的应用及其口腔粘膜再生能力。然而，使用现有方法培养的上皮细胞具有异质性，因此，EVPOME存在细胞生长活性和口腔粘膜再生能力较差的风险。本研究的目的是研究用存在口腔粘膜祖细胞/干细胞富集亚群的小尺寸细胞群制造的EVPOME的能力以及EVPOME皮下移植到小鼠体内后的组织学变化。在由小细胞群制备的EPOME上皮细胞中观察到更多的Ki-67阳性细胞。由小尺寸细胞群制造的 EVPOME 的上皮伸长比使用现有方法的 EVPOME 发生得更快。这些结果表明，具有小细胞群的EVPOME具有高活性和口腔粘膜再生能力。

项目成果

Histochemical aspects of the vascular invasion at the erosion zone of the epiphyseal cartilage in MMP-9-deficient mice

• DOI: 10.2220/biomedres.34.119
• 发表时间: 2013-06-01
• 期刊: BIOMEDICAL RESEARCH-TOKYO
• 影响因子: 1.2
• 作者: Kojima, Taku;Hasegawa, Tomoka;Amizuka, Norio
• 通讯作者: Amizuka, Norio

Remodeling of the Basement Membrane during the Healing Process after Intraoral Grafting of Tissue-Engineered Human Oral Mucosa in Athymic Mice.

无胸腺小鼠口内移植组织工程人类口腔粘膜后愈合过程中基底膜的重塑。

• 发表时间: 2013
• 作者: Yoshizawa M;Koyama T;Funayama A;Mikami T;Kojima T;Niimi K;Kobayashi T;Saito C
• 通讯作者: Saito C

口腔粘膜上皮前駆/幹細胞を応用した培養複合口腔粘膜の皮下移植後の動態

使用口腔粘膜上皮祖细胞/干细胞培养的复合口腔粘膜皮下移植后的动力学

• 发表时间: 2014
• 作者: 芳澤享子;小山貴寛;船山昭典;三上俊彦;小野由起子;小林正治
• 通讯作者: 小林正治

Regeneration Process after Intraoral Grafting of Tissue-Engineered Human Oral Mucosa in Athymic Mice

无胸腺小鼠口内移植组织工程人类口腔粘膜后的再生过程

• 发表时间: 2011
• 期刊: Oral Craniofacial Engineering
• 作者: Yoshizawa M;Koyama T;Funayama A;Mikami T;Saito C
• 通讯作者: Saito C

培養複合口腔粘膜の皮下移植モデルにおける培養上皮の動態

培养复合口腔粘膜皮下移植模型中培养上皮的动态

• 发表时间: 2013
• 作者: Yoshizawa M;Koyama T;Funayama A;Mikami T;Kojima T;Niimi K;Kobayashi T;Saito C;芳澤享子,小山貴寛,船山昭典,三上俊彦,小野由起子,齊藤力
• 通讯作者: 芳澤享子,小山貴寛,船山昭典,三上俊彦,小野由起子,齊藤力