Regeneration of Oral Mucosa by Tissue Engineered Oral Mucosa Grafts.

通过组织工程口腔粘膜移植物实现口腔粘膜再生。

• 批准号: 16390581
• 负责人: YOSHIZAWA Michiko
• 金额: $ 3.78万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16390581/
• 关键词: regenerative medicine; oral mucosa; oral keratinocyte; 上皮細胞; 組織工学

[Introduction] We have developed an ex vivo produced oral mucosa equivalent (EVPOME) in a serum-free culture system without a feeder layer. EVPOME is composed of a stratified layer of oral keratinocytes seeded onto a human Cadaveric dermis, AlloDerm^<【○!R】>. We had started clinical application of EVPOME since 2000. The healing period and the degree of scar formation seemed to be different depend on the sites of grafting. It is necessary to investigate the mechanism of regeneration of oral mucosa by EVPOME in order to obtain a good healing of oral mucosa. The objective of the present study was to develop the animal model of grafted EVPOME on the dorsum skin and in oral cavity and to evaluate the course after EVPOME grafting histologically. [Materials and Methods] The full thickness defects on the dorsum skin or 5mm^2 defects on the buccal mucosa were created in 4-5 week-old nude mice (BALB/c) and EVPOMEs were grafted on the defect. Grafted EVPOMEs were harvested at 5, 7, 14, 21, and 28 days postgrafting for histological examination. As a control, AlloDerm^<【○!R】> was grafted in those defects. [Results] The epithelial coverage was not seen at day 7 postoperatively in both EVPOME and AlloDerm^<【○!R】> grafting on the dorsum skin in the mice. In intraoral grafting group, epithelium was seen on AlloDerm^<【○!R】> as same as EVPOME cultured in vitro at day 5 postoperatively. A discontinuous basal and suprabasal epithelial layer survived, while the superficial keratinized epithelial layer tended to be peeled off AlloDerm^<【○!R】> at day7 postoperatively. Epithelium was more stratified similar to the adjacent mucosa after dayl4 postoperatively. [Discussion and Conclusion] These results suggested that both of the epithelium and basement membrane in EVPOME survived after intraoral grafting and contributed to regeneration of oral mucosa in nude mice, whereas epithelium of EVPOME was not survived after grafting on the dorsum skin.

[简介] 我们开发了一种在无饲养层的无血清培养系统中离体产生的口腔粘膜等效物（EVPOME），EVPOME由接种到人尸体真皮上的分层口腔角质形成细胞组成，AlloDerm^<【○。 !R】> 我们从2000年开始临床应用EVPOME。根据移植部位的不同，愈合时间和疤痕形成程度似乎有所不同。为了获得良好的口腔粘膜愈合效果，有必要研究EVPOME对口腔粘膜再生的机制。本研究的目的是建立背部皮肤和口腔中移植EVPOME的动物模型并进行评估。 EVPOME移植后病程组织学观察 [材料与方法] 4-5小时制作背部皮肤全层缺损或颊黏膜5mm^2缺损。将一周龄裸鼠(BALB/c)和EVPOME移植到移植后5、7、14、21和28天，用于组织学检查，AlloDerm^<【○!R。 ]> 在这些缺损处进行了移植 [结果] 术后第 7 天，EVPOME 和 EVPOME 均未见上皮覆盖。 AlloDerm^<【○!R】>移植到小鼠的背部皮肤上，口内移植组术后第5天，在AlloDerm^<【○!R】>上看到与体外培养的EVPOME相同的上皮，不连续。基底层和基底上皮层存活，而浅层角化上皮层趋于剥落 AlloDerm^<【○!R】>术后第7天，上皮与术后第14天的邻近粘膜更加分层。 [讨论与结论]这些结果表明EVPOME在口内移植后均存活并有助于裸鼠口腔粘膜的再生。而EVPOME移植到背部皮肤后上皮没有存活。