SNP typing of the cariogenic protein gene

致龋蛋白基因的SNP分型

• 批准号: 23592790
• 负责人: MATSUDA Yasuhiro
• 金额: $ 3.16万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23592790/
• 关键词: 遺伝子多型; 遺伝子分析; う蝕感受性; 唾液緩衝能; 齲蝕; PCR高解像度融解曲線分析

To evaluate the discrimination method of HLA Alleles, we developed the detection method by PCR-Sequence Specific Primer (SSP) and the melt peak analysis (Tm). The frequencies of target Allele calculated from database were less than 10%, therefore our method could discriminate more than 90% samples.We analyzed the Buffer capacity of saliva and Carbonic Anhydrase VI SNP of Children attended permanent caries preventing program. There were a positive association between caries present and buffer capacity. We could distinguish the rs2274327 (C/T) polymorphism by PCR-Restriction Fragment Length Polymorphism (PCR-RFLP) method.

为了评估HLA等位基因的辨别方法，我们开发了PCR-序列特异性引物（SSP）和熔解峰分析（Tm）的检测方法。从数据库计算出的目标等位基因的频率小于10%，因此我们的方法可以区分超过90%的样本。我们分析了参加永久防龋项目的儿童的唾液缓冲能力和碳酸酐酶VI SNP。龋齿的存在与缓冲能力之间存在正相关关系。通过PCR-限制性片段长度多态性(PCR-RFLP)方法可以区分rs2274327(C/T)多态性。

项目成果

Comparison of Fluoride Regimens on Fluorine Uptake in Carious Enamel

氟化物方案对龋齿釉质氟吸收的比较

• 发表时间: 2013
• 作者: Naoki HASHIMOTO;Yoshiki FUNATO;Saiko OKI;Yasuhiro. MATSUDA;Katsushi OKUYAMA;Hiroko YAMAMOTO;Hisanori KOMATSU;and Hidehiko SANO
• 通讯作者: and Hidehiko SANO

Effective Screening for the HLA-B*35:05 and HLA-B*57:01 Alleles by Using an Allele-Specific Polymerase Chain Reaction Melting Assay

使用等位基因特异性聚合酶链反应熔解测定有效筛选 HLA-B*35:05 和 HLA-B*57:01 等位基因

• 发表时间: 2014
• 期刊: 2013 International Conference on Biological, Medical and Chemical Engineering (BMCE 2013)
• 作者: Yasuhiro Matsuda;Hidehiko Sano;Yuichi Iwaki
• 通讯作者: Yuichi Iwaki

う蝕と遺伝子解析

龋齿与遗传分析

• 发表时间: 2013
• 期刊: 北海道歯誌
• 作者: Han L;Kodama S;Okiji T;Kamada A.;松田康裕
• 通讯作者: 松田康裕

Effective Screening for the HLA-B*35: 05 and HLA-B*57: 01 Alleles by Using an Allele-Specific Polymerase Chain Reaction Melting Assay

使用等位基因特异性聚合酶链式反应熔解测定有效筛选 HLA-B*35: 05 和 HLA-B*57: 01 等位基因

• 发表时间: 2013
• 期刊: 2013 International Conference on Biological, Medical and Chemical Engineering (BMCE 2013)
• 作者: Yasuhiro Matsuda;Hidehiko Sano;Yuichi Iwaki
• 通讯作者: Yuichi Iwaki

Demineralize Prevention of Dentin With Fluoride Varnish via Automatic pH-cycling

通过自动 pH 循环使用氟化物清漆预防牙本质脱矿质

• 发表时间: 2013
• 作者: Saiko OKI;Yasuhiro MATSUDA;Naoki HASHIMOTO;Katsushi OKUYAMA;Hiroko YAMAMOTO;Yoshiki FUNATO;Hisanori KOMATSU;and Hidehiko SANO
• 通讯作者: and Hidehiko SANO