Establishment of new concepts in cone electroretinogram using transgenic rabbits

转基因兔锥体视网膜电图新概念的建立

• 批准号: 23592603
• 负责人: KONDO MINEO
• 金额: $ 3.24万
• 依托单位: Mie University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23592603/
• 关键词: 網膜色素変性; 網膜電図; 動物モデル; トランスジェニック; 錐体; ウサギ; ロドプシン

The purpose of this study was to determine the contributions of cones and post- photoreceptoral neurons to the a-wave of the photopic ERGs in rhodopsin Pro347Leu transgenic (Tg) rabbits. Post-photoreceptral neural function was completely suppressed by intravitreal injections of CNQX and APB. We found that the percentage contribution of the cone photoreceptorsto the photopic ERG a-waves ranged from 54% to 75% in wild-type (WT) rabbits. In contrast, the percentage contribution of the cone photoreceptors in Tg rabbits ranged from 32% to 51%. These results suggest that the relative contribution of cone photoreceptors to the photopic ERG a-wave is smaller in retinas with inherited photoreceptor degeneration.

这项研究的目的是确定视锥神经元和光感受体后神经元对Rhodopsin Pro347Leu转基因（TG）兔子中光的A波的贡献。玻璃体室内注射CNQX和APB完全抑制了鼻部后神经功能。我们发现，圆锥体光感受器的百分比贡献在野生型兔（WT）兔中的光接ERG A波的百分比为54％至75％。相比之下，TG兔子中锥形感受器的百分比贡献范围为32％至51％。这些结果表明，在带有遗传感受器变性的视网膜中，锥光受体对光波ERG A波的相对贡献较小。

项目成果

ISCEV standard for clinical multifocal electroretinography (mfERG) (2011 edition).

• DOI: 10.1007/s10633-011-9296-8
• 发表时间: 2012-02
• 期刊: DOCUMENTA OPHTHALMOLOGICA
• 影响因子: 1.4
• 作者: Hood, Donald C.;Bach, Michael;Brigell, Mitchell;Keating, David;Kondo, Mineo;Lyons, Jonathan S.;Marmor, Michael F.;McCulloch, Daphne L.;Palmowski-Wolfe, Anja M.
• 通讯作者: Palmowski-Wolfe, Anja M.

A naturally occurring canine model of autosomal Recessive complete-type congenital stationary night blindness

自然发生的常染色体隐性完全型先天性静止性夜盲症犬模型

• 发表时间: 2013
• 作者: Kondo M;Imai R;Nakashita T;Imawaka M;Ueda K;Ohtsuka H;Sakai K;Matsubara H;Sugimoto M;Ueno S;Nishizawa Y;Das G;Miyadera K;Aguirre G
• 通讯作者: Aguirre G

Enhancement of ON-bipolar cell responses of cone electroretinograms in rabbits with the Pro347Leu rhodopsin mutation.

Pro347Leu 视紫红质突变兔锥体视网膜电图 ON-双极细胞反应的增强。

• DOI: 10.1167/iovs.11-7611
• 发表时间: 2011
• 期刊: Invest. Ophthalmol. Vis. Sci.
• 作者: Nishimura T;Machida S;Kondo M;Terasaki H;Yokoyama D;Kurosaka D.
• 通讯作者: Kurosaka D.

Transient increase of retinal nerve fiber layer thickness after macular hole surgery

• DOI: 10.1007/s10792-013-9855-y
• 发表时间: 2014-06-01
• 期刊: INTERNATIONAL OPHTHALMOLOGY
• 影响因子: 1.6
• 作者: Hibi, Nobuaki;Kondo, Mineo;Terasaki, Hiroko
• 通讯作者: Terasaki, Hiroko

Focal macular electroretinograms after intravitreal injections of bevacizumab for age-related macular degeneration.

玻璃体内注射贝伐单抗治疗年龄相关性黄斑变性后的局灶性黄斑视网膜电图。

• DOI: 10.1167/iovs.11-9335
• 发表时间: 2012
• 期刊: Invest Ophthalmol Vis Sci.
• 作者: 中島史絵;重安千花;藤井かんな;井之川宗右;阿久根陽子;山田昌和;Iwata E
• 通讯作者: Iwata E