Establishment of rhodopsin transgenic rabbit line and analysis of retinal degeneration

视紫红质转基因兔系的建立及视网膜变性分析

• 批准号: 20592075
• 负责人: KONDO Mineo
• 金额: $ 2.91万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20592075/
• 关键词: 網膜色素変性; 動物モデル; ウサギ; 網膜電図; トランスジェニック; 網膜変性; ロドプシン; 視細胞

We have generated a rabbit model of retinitis pigmentosa (RP), the rhodopsin P347L transgenic (Tg) rabbit, by using bacterial artificial chromosome (BAC) transgenesis. These rabbits showed a rod-dominant, progressive retinal degeneration with a marked regional variations in the loss of photoreceptors. All of the electroretinogram components of the Tg rabbits decrease progressively with the a-wave more affected than the b-wave, and the oscillatory potentials (OPs) were the best preserved. Interestingly, the OP amplitudes of young Tg rabbits were significantly larger than those of wild-type rabbits. Pharmacological experiments showed that the significantly larger OPs in young Tg rabbits resulted from secondary alterations in the inner retinal function.

我们通过使用细菌性人造染色体（BAC）转基因生成了色素性视网膜炎（RP），呈视紫红质p347l转基因（TG）兔的兔模型。这些兔子表现出杆位主导的，进行性视网膜变性，具有显着的光感受器损失的区域变化。 TG兔子的所有电视图组件随着A波的影响逐渐减少，而振荡电势（OPS）保存最佳。有趣的是，年轻的TG兔子的运行幅度明显大于野生型兔子。药理学实验表明，年轻TG兔子的OPS明显较大，这是由于内部视网膜功能的次要变化而产生的。

项目成果

Efficacy of Suprachoroidal-Transretinal Stimulation in Rabbit Model of Retinal Degeneration

脉络膜上-经视网膜刺激对兔视网膜变性模型的疗效

• 发表时间: 2010
• 期刊: Invest Ophthalmol Vis Sci 51
• 作者: Nishida K;Kamei M;Kondo M;Sakaguchi H;Suzuki M;Fujikado T;Tano Y
• 通讯作者: Tano Y

Blimpl suppresses Chx10 expression in differentiating retinal photoreceptor precursors to ensure proper photoreceptor development

Bimplimpl 抑制分化视网膜光感受器前体细胞中的 Chx10 表达，以确保光感受器正常发育

• 发表时间: 2010
• 期刊: Journal of Neuroscience
• 影响因子: 5.3
• 作者: Katoh K;Omori Y;Onishi A;Sato S;Kondo M;Furukawa T
• 通讯作者: Furukawa T

Gene expression analysis of embryonic photoreceptor precursor cells using BAC-Crx-EGFP transgenic mouse

• DOI: 10.1016/j.bbrc.2010.01.007
• 发表时间: 2010-02-12
• 期刊: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
• 影响因子: 3.1
• 作者: Muranishi, Yuki;Sato, Shigeru;Furukawa, Takahisa
• 通讯作者: Furukawa, Takahisa

TRPM1 is a component of the retinal ON bipolar cell transduction channel in the mGluR6 cascade

• DOI: 10.1073/pnas.0912730107
• 发表时间: 2010-01-05
• 期刊: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• 影响因子: 11.1
• 作者: Koike, Chieko;Obara, Takehisa;Furukawa, Takahisa
• 通讯作者: Furukawa, Takahisa

Asymmetry of focal macular photonic negative responses (PhNRs) in monkeys.

猴子黄斑局灶光子负反应（PhNR）的不对称性。

• 发表时间: 2008
• 期刊: Experimental Eye Research 88
• 作者: Kurimoto Y;Kondo M;Ueno S;Sakai T;Machida S;Terasaki H
• 通讯作者: Terasaki H