Mechanisms of specific regulation of gonadotropn subunits expression by pulsatile GnRH stimulation

脉冲式 GnRH 刺激特异性调节促性腺激素亚基表达的机制

• 批准号: 23592404
• 负责人: KANASAKI Haruhiko
• 金额: $ 3.24万
• 依托单位: Shimane University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23592404/
• 关键词: GnRH; ゴナドトロピン; ＧｎＲＨ; TSA; FSH; PACAP

PACAP and its receptor are expressed not only in the central nervous system, but also in peripheral organs and function to stimulate pituitary hormone synthesis and release as both hypothalamic-pituitary releasing factor as well as an autocrine-paracrine factor within the pituitary. PACAP stimulates the expression of the gonadotropin alpha, LH betand FSH beta subunits as well as the GnRH receptor and its own PACAP type I receptor (PAC1R) in gonadotropin-secreting pituitary cells. In turn, GnRH, which has been known as a crucial component of gonadotropin secretion, stimulates the expression of PACAP and PAC1R in gonadotrophs. In addition, PAC1R presents in GnRH-producing neuronal cells and PACAP modulates the functions of GnRH neurons. It is evident that PACAP plays a role in reproductive function through regulating gonadotropins directly or indirectly by modulating GnRH release.

PACAP及其受体不仅在中枢神经系统中表达，而且在周围器官中也表达了刺激垂体激素合成和释放的功能，并作为下丘脑垂体释放因子以及垂体内的自身分泌因子。 PACAP刺激了促性腺激素分泌垂体细胞中促性腺激素α，LH Betand FSHβ亚基以及GNRH受体的表达及其自己的PACAP I型受体（PAC1R）。反过来，GnRH被称为促性腺激素分泌的关键成分，刺激了促性腺营养性PACAP和PAC1R的表达。此外，PAC1R在产生GNRH的神经元细胞中呈现，PACAP调节GNRH神经元的功能。显然，PACAP通过调节GNRH释放直接或间接调节促性腺激素在生殖功能中起作用。

项目成果

Expression of the pituitary adenylate cyclase-activating polypeptide (PACAP) type 1 receptor (PAC1R) potentiates the effects of GnRH on gonadotropin subunit gene expression.

• DOI: 10.1016/j.bbrc.2011.05.135
• 发表时间: 2011-07
• 期刊: Biochemical and biophysical research communications
• 影响因子: 3.1
• 作者: I. Purwana;H. Kanasaki;A. Oride;T. Mijiddorj;K. Miyazaki

Prolonged stimulation with thyrotropin-releasing hormone and pituitary adenylate cyclase-activating polypeptide desensitize their receptor functions in prolactin-producing GH3 cells

促甲状腺激素释放激素和垂体腺苷酸环化酶激活多肽的长期刺激可使其在催乳素产生的 GH3 细胞中的受体功能脱敏

• 发表时间: 2013
• 期刊: Mol Cell Endocrinol
• 影响因子: 4.1
• 作者: Mijiddorj T;Kanasaki H;Sukhbaatar U;Oride A;Purwana IN;Miyazaki K
• 通讯作者: Miyazaki K

Possible role of PACAP and its PAC1 receptor in the differential regulation of pituitary LHβ- and FSHβ- subunit gene expression by pulsatile GnRH stimulation

PACAP 及其 PAC1 受体在脉动 GnRH 刺激对垂体 LHβ 和 FSHβ 亚基基因表达的差异调节中的可能作用

• 发表时间: 2013
• 期刊: Biol Reprod
• 影响因子: 3.6
• 作者: Kanasaki H;Purwana IN;Miyazaki K
• 通讯作者: Miyazaki K

Follistatin, induced by thyrotropin-releasing hormone (TRH), plays no role in prolactin expression but affects gonadotropin FSHb expression as a paracrine factor in pituitary somatolactotroph GH3 cells

卵泡抑素由促甲状腺激素释放激素 (TRH) 诱导，在催乳素表达中不起任何作用，但会影响促性腺激素 FSHb 作为垂体促泌乳素细胞 GH3 细胞中的旁分泌因子的表达

• 发表时间: 2010
• 作者: Kanasaki H;Oride A;Purwana IN;Miyazaki K
• 通讯作者: Miyazaki K

卵子の成熟に果たす下垂体LH, FSH産生の意義

垂体 LH 和 FSH 产生对卵母细胞成熟的意义

• 发表时间: 2011
• 作者: 金崎春彦;プルワナ インドリ;折出亜希;ミジドルジ ツェルメグ;宮崎康二
• 通讯作者: 宮崎康二