Regulation of reactive oxygen species induced by heavy-ion (carbon) beam; Pursuing the sophistication of heavy-ion cancer therapy

重离子（碳）束诱导的活性氧的调节；

• 批准号: 23591853
• 负责人: MATSUMOTO Ken-ichiro
• 金额: $ 3.24万
• 依托单位: National Institute of Radiological Sciences
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23591853/
• 关键词: 重粒子線がん治療; 抗酸化剤; レドックス制御; 活性酸素; フリーラジカル; 放射線; 重粒子線; ヒドロキシルラジカル; スーパーオキサイド; 過酸化水素; 電子常磁性共鳴

Density of radiation induced hydroxyl radical could be measured by electron paramagnetic resonance (EPR) spin trapping method. Superoxide could be measured by EPR spin probing method. Hydrogen peroxide could also be measured by EPR spin probing method. Results from those measurements gave a prediction that the very dense hydroxyl radical generation by high LET radiation can not be regulated by biologically available antioxidants concentration. Other reactive oxygen species including low density hydroxyl radical could possibly be regulated or partly regulated using antioxidants.Inhibitory effect of TEMPOL to the radiation induced fibrosis caused by carbon-ion beam on mouse leg muscle was tested. Administration of TEMPOL dose affect to the fibrosis contrary to expectations.

辐射诱导的羟基自由基的密度可以通过电子顺磁共振（EPR）自旋捕获方法来测量。超氧化物可以通过EPR自旋探测法来测量。过氧化氢也可以通过 EPR 旋转探测法测量。这些测量结果预测，高 LET 辐射产生的非常密集的羟基自由基不能通过生物可用的抗氧化剂浓度来调节。其他活性氧，包括低密度羟自由基，可能可以通过抗氧化剂进行调节或部分调节。测试了TEMPOL对碳离子束引起的小鼠腿部肌肉辐射诱导纤维化的抑制作用。 TEMPOL剂量对纤维化的影响与预期相反。

项目成果

Density of Hydroxyl Radical Generated in an Aqueous Solution Irradiated by Carbon Ion Beam

碳离子束照射水溶液中产生的羟基自由基的密度

• DOI: 10.1016/j.freeradbiomed.2012.08.429
• 发表时间: 2012
• 期刊: Free Radical Biology & Medicine
• 影响因子: 7.4
• 作者: 吉田（橋立）智美;清水孝雄;中村元直;横山智史;松本謙一郎
• 通讯作者: 松本謙一郎