A basic study for evaluation method of brain function on forensic autopsy cases.

法医尸检案件脑功能评价方法的基础研究

• 批准号: 23590851
• 负责人: NISHIMURA Akiyoshi
• 金额: $ 3.33万
• 依托单位: The University of Tokushima
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23590851/
• 关键词: 法医病理学; dementia; schizophrenia; hippocampus; lectin; erythropoietin receptor; NOS; N-Cadherin; chondroitin sulfate; Alzheimer; Braak stage; dopaminergic; serotonin; cholinergic; microglia; astrocyte; レクチン; アルツハイマー; Gallias-Braak; 海馬歯状回

Autopsied brains with dementia were examined by means of immunohistochemical staining method using 17 antibodies, related with neuronal degeneration, cellular damage and oxidative stress. Especially, a large number of SPDs were stained by antibodies of YKL-39, Tau, CS and HSP70. Furthermore, development frequency of SPDs between by mean of NOS and GFAP, NOS and CS, ApoE and CS, Tau and HSP70, and CS and HSP70, showed positive correlations. Substances relating with dementia are detected in same SPD by mean of multi fluorescent immunehistochemical staining visualized by confocal laser microscopy. Although, SPD were related with apoptosis of neurons in schizophrenia, SPD might come from not only neuronal degeneration but also glial in degenerative dementia. SPDs with reactivity of EAAC1 and/or alpha-syn related with neuronal degeneration were detected in the early stages and they with reactivity of S-100 and/or YKL-40 related with astrocytes were detected in the middle or later stages.

使用17种与神经元变性，细胞损伤和氧化应激有关的抗体，通过免疫组织化学染色方法检查了具有痴呆症的大脑。特别是，大量SPD被YKL-39，TAU，CS和HSP70的抗体染色。此外，通过NOS和GFAP，NOS和CS，APOE和CS，TAU和HSP70以及CS和HSP70之间的SPD的发展频率显示为正相关。通过共聚焦激光显微镜可视化的多荧光免疫组织化学染色，在同一SPD中检测到与痴呆有关的物质。尽管SPD与精神分裂症中神经元的凋亡有关，但SPD不仅来自神经元变性，而且还来自退行性痴呆中的神经胶质。在早期阶段检测到具有与神经元变性有关的EAAC1和/或α-Syn反应性的SPD，它们在中间或后期检测到与星形胶质细胞相关的S-100和/或YKL-40的反应性。

项目成果

ヒト剖検脳の海馬歯状回分子層における球状沈着物の免疫組織化学的検討

人体尸检脑海马齿状回分子层球形沉积物的免疫组织化学研究

• 发表时间: 2013
• 作者: 山田茉樹;石上安希子;徳永逸夫;西村明儒
• 通讯作者: 西村明儒

Histopathological investigations of lectin positive spherical deposits detected in the molecular layer of the hippocampal formation with neurofibrillary tangles

在海马结构分子层检测到的凝集素阳性球形沉积物与神经原纤维缠结的组织病理学研究

• 发表时间: 2014
• 作者: Akiyoshi NISHIMURA;Akiko ISHIGAMI,Hiromitsu;KURATA;Itsuo TOKUNAGA
• 通讯作者: Itsuo TOKUNAGA

Histopathological investigations of lectin positive spherical deposits detected in the molecular layer of the hippocampal formation with neurofibrillary tangles.

在海马结构分子层中检测到的凝集素阳性球形沉积物与神经原纤维缠结的组织病理学研究。

• 发表时间: 2014
• 作者: Akiyoshi NISHIMURA;Akiko ISHIGAMI，Hiromitsu;KURATA;Itsuo TOKUNAGA
• 通讯作者: Itsuo TOKUNAGA