Development of immobilized koji-mold catalyst for effective isolation of pharmaceutically significant yerba mate compounds

开发用于有效分离药学上重要的马黛茶化合物的固定曲霉催化剂

• 批准号: 23580135
• 负责人: ADACHI OSAO
• 金额: $ 3.49万
• 依托单位: Yamaguchi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23580135/
• 关键词: マテ茶成分; 麹菌触媒; 酢酸菌; 酸化発酵; シキミ酸; タミフル; キナ酸の製造; シキミ酸の製造; マテ茶の薬効成分; 固定化触媒; マテ茶有効成分; 固定化麹触媒法; アルゼンチン; クロロゲン酸; クロロゲン酸水解酵素; マテ茶; キナ酸; カフェ酸

A novel strategy preparing raw materials for pharmaceuticals such as shikimic acid for Tamiflu was established applying koji-mold catalyst to yerba mate tea. Chlorogenic acid (CGA) hydrolase (CGase) was potently induced in mycelia such as Aspergillus niger when grown on yerba mate extract. Heat treatment of mycelia at 40˚C for 30 min destroyed polyphenol oxidase but CGase was highly resistant and survived during the heat treatment.Thus, the heated mycelia were found useful as a kind of immobilized catalyst of CGase. Some model reaction using immobilized heated mycelia as CGase was studied. This is the most outstanding point in this study and this method can be accepted for industrial production of QA and caffeic acid (CA). QA can be converted to shikimate by two enzyme systems of acetic acid bacteria, as has already been established in the previous study. Simultaneous isolation of QA, CGA, and CA was established by a simple ion exchange column chromatography.

一种新型的药物疗法，例如叶绿酸。但是CGase具有高度抗性，并在theatmet中幸存下来。乙酸的酶系统，如先前的研究中已经建立的。

项目成果

Pentose oxidation by acetic acid bacteria led to a finding of membrane-bound purine nucleosidase

乙酸细菌的戊糖氧化导致膜结合嘌呤核苷酶的发现

• 发表时间: 2013
• 期刊: Biosci. Biotechnol. Biochem
• 作者: Adachi O;Hours RA;Akakabe Y;Shinagawa E ;Ano Y;Yakushi T;& Matsushita K.
• 通讯作者: & Matsushita K.

4-ケトリボ酸、4-ケトリボース、及びそれらの製造方法

4-酮二糖酸、4-酮二糖及其制备方法

• 发表时间: 2011

Enzymatic synthesis of 4-pentulosonate (4-keto-D- pentonate) from D-aldopentose and D-pentonate with two different pathways using membrane enzymes of acetic acid bacteria

使用乙酸菌膜酶通过两种不同途径从 D-戊醛糖和 D-戊糖酸酶法合成 4-戊酮酸（4-酮-D-戊糖酸）

• 发表时间: 2011
• 期刊: Biosci. Biotechnol. Biochem
• 作者: Adachi O;Hours RA;Shinagawa E;Akakabe Y;Yakushi T;& Matsushita K.
• 通讯作者: & Matsushita K.

4-Keto-D-aldopentoses and 4-keto-pentulosonates, new products with acetic acid bacteria

4-酮-D-戊醛糖和4-酮-戊酮酸，醋酸菌新产品

• 发表时间: 2012
• 期刊: Acetic Acid Bacteria, 1
• 作者: Adachi O;Hours RA;Shinagawa E;Akakabe Y;Yakushi T;& Matsushita K.
• 通讯作者: & Matsushita K.

Characterizacion de una clorogenato hydrolase de Aspergillus niger AKU 3302 inducida con extractos de yerba mate

黑曲霉 AKU 3302 与马黛茶提取物诱导的绿原水解酶的表征

• 发表时间: 2014
• 作者: Butiuk AP;Maidana AS;Adachi O;& Hours RA.
• 通讯作者: & Hours RA.