Controlled release of growth factors for bone regeneration

控制释放生长因子以促进骨再生

• 批准号: 23500543
• 负责人: TACHIBANA Akira
• 金额: $ 3.33万
• 依托单位: Osaka City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23500543/
• 关键词: 徐放システム; FGF2; キチン; キチンゲル; キチン結合ドメイン; リゾチーム応答性徐放; chitin binding domain; BMP2/7; キトサン; セルロース; セルロース結合ドメイン; pH感受性

Chitin gel was obtained by the acetylation of chitosan in solution. Basic growth factors did not bind to non treated chitin gel, whereas tightly bound to carboxymethylated chitin gel. Chitin binding domain-FGF-2 was bound to chitin gel and not released by PBS washing. Chitin binding domain-FGF-2 was released by degradation of chitin gel with lysozyme. During degradation of chitin gel with lysozyme, chitin fragment was released at a burst, but chitin binding domain-FGF-2 was not. Most of chitin binding domain-FGF-2 that was released from gel were re-bound to the gel, only low amount of FGF-2 was released out. Therefore, ideal controlled release without a burst release was realized.

通过溶液中壳聚糖的乙酰化获得壳蛋白凝胶。基本生长因子与未处理的几丁质凝胶没有结合，而与羧甲基化的几丁质凝胶紧密结合。几丁质结合结构域-FGF-2与几丁质凝胶结合，而不是通过PBS洗涤释放。几丁质结合结构域-FGF-2通过用溶菌酶降解粒蛋白凝胶释放。在用溶菌酶降解的粒蛋白凝胶降解期间，几丁质片段在爆发中释放出来，但几丁质结合结构域-FGF-2却没有。从凝胶中释放的大多数几丁质结合域FGF-2都重新结合到凝胶上，仅释放了少量的FGF-2。因此，实现了理想的受控释放，而没有突发释放。

项目成果

キチンゲルを用いたFGF2の徐放

使用甲壳素凝胶持续释放 FGF2

• 发表时间: 2012
• 作者: 安間 大;立花 亮;田辺 利住
• 通讯作者: 田辺 利住

キチンゲルを用いたFGF2の徐放/Controlled release of FGF2 using chitin gel

使用甲壳素凝胶控制 FGF2 的释放

• 发表时间: 2012
• 作者: 安間大;立花亮;田辺利住
• 通讯作者: 田辺利住

キチンゲルを用いたFGF2の徐放/Controlled release of FGF2 using chitin gel バイオインターフェース先端マテリアルの創生

使用甲壳素凝胶控制释放 FGF2 创建先进的生物界面材料

• 发表时间: 2013
• 作者: 安間大;立花亮;田辺利住
• 通讯作者: 田辺利住