Diagnostic methods to predict the development of autoimmune diseases by gene polymorphisms of immune regulatory factors

利用免疫调节因子基因多态性预测自身免疫性疾病发生的诊断方法

• 批准号: 23390150
• 负责人: YOSHINORI Iwatani
• 金额: $ 11.81万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23390150/
• 关键词: 免疫応答制御因子; 遺伝子多型; 自己免疫疾患; 発症予知診断法; 疾患感受性遺伝子; サイトカイン; DNAメチル化酵素; ＣＤ２４; ＩＬ－１０; ＥＰＯ; 一塩基多型; 発症予知; 重症度; CD40; FCRL3; DNMT1; MTRR; マスター遺伝子; Th1細胞; Th2細胞

In order to develop the predictive diagnostic methods for the development and prognosis of autoimmune thyroid diseases, we analyzed the single mononucleotide polymorphisms (SNPs) of immune regulatory factors in patients with Hashimoto's disease and with Graves' disease. We clarified the usefulness of ZFAT Ex9b-SNP10T/A, MTRR +66A/G, IL-10 -592A/C and TSHR rs179247A/G SNPs to predict the development of hypothyroidism under 50 years old in patients with Hashimoto's disease, and that of TBX21 -1514T/C, HLX -742C/G, IL-13 -1112C/T, CD40 -1C/T,FCRL3 -169C/T, DNMT1 +32204A/G and TSHR rs179247A/G SNPs to predict the intractability of Graves' disease against anti-thyroid drug therapy. Furthermore, we clarified the importance of an EPO -1125G/T SNP which determine the strength of angiogenic capability for the development of Graves' disease. These data indicate that the SNPs of immune regulatory factors are useful for the predictive diagnostic methods for autoimmune diseases.

为了开发自身免疫性甲状腺疾病的发生和预后的预测诊断方法，我们分析了桥本病和格雷夫斯病患者免疫调节因子的单核苷酸多态性（SNP）。我们阐明了 ZFAT Ex9b-SNP10T/A、MTRR +66A/G、IL-10 -592A/C 和 TSHR rs179247A/G SNP 在预测 50 岁以下桥本病患者甲状腺功能减退症发展方面的有用性，以及TBX21 -1514T/C、HLX -742C/G、IL-13 -1112C/T、CD40 -1C/T、FCRL3 -169C/T、DNMT1 +32204A/G 和 TSHR rs179247A/G SNP 可预测 Graves 病对抗甲状腺药物治疗的难治性。此外，我们阐明了 EPO -1125G/T SNP 的重要性，它决定了格雷夫斯病发展中血管生成能力的强度。这些数据表明免疫调节因子的SNP对于自身免疫性疾病的预测诊断方法是有用的。

项目成果

Prolonged hybridization with a cRNA probe improves the signal to noise ratio for in-tube in situ hybridization for quantification of mRNA after fluorescence-activated cell sorting

与 cRNA 探针的长时间杂交可提高荧光激活细胞分选后用于定量 mRNA 的管内原位杂交的信噪比

• DOI: 10.3109/10520295.2012.672650
• 发表时间: 2012
• 期刊: Biotech Histochem
• 影响因子: 1.6
• 作者: Yamada;H.;Yamakawa;N.;Watanabe;M.;Hidaka;Y.;Iwatani;Y.;Takano;T.
• 通讯作者: T.

Different genotypes of a functional polymorphism of the TSHR gene are associated with the development and severity of Graves and Hashimotos diseases

TSHR 基因功能多态性的不同基因型与格雷夫斯病和桥本病的发生和严重程度相关

• DOI: 10.1111/tan.12190
• 发表时间: 2013
• 期刊: Tissue Antigen
• 作者: Inoue;N.;Watanabe;M.;Katsumata;Y.;Hidaka;Y.;Iwatani;Y
• 通讯作者: Y

Interference by Pralidoxime (PAM) salts in clinical laboratory tests

解磷定 (PAM) 盐对临床实验室测试的干扰

• DOI: 10.1016/j.cca.2012.11.017
• 发表时间: 2013
• 期刊: Clin Chim Acta
• 影响因子: 5
• 作者: Nagase;S.;Kohguchi;K.;Tohyama;K.;Watanabe;M.;Iwatani;Y
• 通讯作者: Y

IL-10 -592A/C polymorphism is associated with severity of Hashimoto's disease

IL-10 -592A/C 多态性与桥本氏病的严重程度相关

• DOI: 10.1016/j.cyto.2013.05.014
• 发表时间: 2013
• 期刊: Cytokine
• 影响因子: 3.8
• 作者: Inoue;N.;Watanabe;M.;Wada;M.;Morita;M.;Hidaka;Y.;Iwatani;Y
• 通讯作者: Y

Functional polymorphisms in TBX21 and HLX are associated with development and prognosis of Graves' disease

• DOI: 10.3109/08916934.2011.622013
• 发表时间: 2012-03-01
• 期刊: AUTOIMMUNITY
• 影响因子: 3.5
• 作者: Morita, Mami;Watanabe, Mikio;Iwatani, Yoshinori
• 通讯作者: Iwatani, Yoshinori