Elucidation of modification mechanism of secondary metabolites in plant vacuoles.

阐明植物液泡中次生代谢产物的修饰机制。

• 批准号: 23370016
• 负责人: OZEKI Yoshihiro
• 金额: $ 11.73万
• 依托单位: Tokyo University of Agriculture and Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23370016/
• 关键词: アントシアニン; 配糖化酵素; アシル化酵素; 液胞; Zwitter donor; p-hydroxybenzyl-glucose; デルフィニウム; Delphinium grandiflorum; シロイヌナズナ; アシルグルコース; glycoside hydrolase family 1; シネラリア; アガパンサス; アシル-グルコース

Acyl-glucose dependent glucosyltransfrase (AAGT) gene was found in a monocot plant, Agapanthus, and Arabidopsis AAGT acted to acyl-modified anthocyanin with glucosyl residues in red turning leaves, indicating that AAGT reaction is common in the plant kingdom. In delphinium sepals, p-hydroxybenzyl-glucose (pHBG) acted as a glucosyl-donor at modification at the 7 position of anthocyanidin. The anthocyanin, violdelphin, consisted of three glycosyl and two p-hydroxybenzyl (pHBA)-residues, conjugated with alternately tandem and linear conjugation of glycosyl and pHBA-residues. In the reaction to form violdelphin, it was revealed that pHBG played a bi-functional role to be acyl donors and glucosyl donors for acyl-glucose dependent acyl-transferase and AAGT as Zwitter donor. The gene for UDP-glucose dependent pHBA glucosyltransferase was identified and the loss of this activity resulted not to modify at the 7 position of anthocyanidin.

在单子叶植物百子莲和拟南芥中发现酰基葡萄糖依赖性葡萄糖基转移酶（AAGT）基因，AAGT作用于红转叶中带有葡萄糖基残基的酰基修饰花青素，表明AAGT反应在植物界很常见。在飞燕草萼片中，对羟基苄基葡萄糖 (pHBG) 在花青素的 7 位修饰上充当葡萄糖基供体。花青素 violdelphin 由三个糖基和两个对羟基苯甲基 (pHBA) 残基组成，通过糖基和 pHBA 残基交替串联和线性缀合进行缀合。在形成紫海豚的反应中，pHBG 发挥双功能作用，作为酰基葡萄糖依赖性酰基转移酶的酰基供体和葡萄糖基供体，AAGT 作为两性供体。鉴定了 UDP 葡萄糖依赖性 pHBA 葡萄糖基转移酶基因，该活性的丧失导致花青素的 7 位不发生修饰。

项目成果

Rice Os9BGlu31 Is a Transglucosidase with the Capacity to Equilibrate Phenylpropanoid, Flavonoid, and Phytohormone Glycoconjugates

• DOI: 10.1074/jbc.m112.423533
• 发表时间: 2013-04-05
• 期刊: JOURNAL OF BIOLOGICAL CHEMISTRY
• 影响因子: 4.8
• 作者: Luang, Sukanya;Cho, Jung-Il;Cairns, James R. Ketudat
• 通讯作者: Cairns, James R. Ketudat

Formation of volatile halogenated compounds in fresh-cut cabbage treated with sodium hypochlorite.

用次氯酸钠处理的鲜切卷心菜中挥发性卤代化合物的形成。

• 发表时间: 2012
• 期刊: Jpn. J. Food Chem. Safety
• 作者: Kubota;H.
• 通讯作者: H.

Comparison of signal enhancement techniques using DNA microarrays for screening GM crops.

使用 DNA 微阵列筛选转基因作物的信号增强技术的比较。

• 发表时间: 2013
• 期刊: Jpn. J. Food Chem. Safety
• 作者: Ito;A.
• 通讯作者: A.

赤系デルフィニウムのアントシアニンの構造と F3’5’H 遺伝子の解析

红飞燕草花色苷结构及F35H基因分析

• 发表时间: 2013
• 作者: 宮川菜月;西崎雄三;宮原 平;廣瀬由起夫;岡本充智;小関良宏;佐々木伸大
• 通讯作者: 佐々木伸大

Determination of sodium stearoyl lactylates in foods using HPLC after derivatization with 2-nitrophenyl hydrazine.

采用 2-硝基苯肼衍生化后的 HPLC 法测定食品中的硬脂酰乳酸钠。

• 发表时间: 2013
• 期刊: Jpn. J. Food Chem. Safety
• 作者: Mikawa;T.
• 通讯作者: T.