Elucidation of modification mechanism of secondary metabolites in plant vacuoles.

阐明植物液泡中次生代谢产物的修饰机制。

• 批准号: 23370016
• 负责人: OZEKI Yoshihiro
• 金额: $ 11.73万
• 依托单位: Tokyo University of Agriculture and Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23370016/
• 关键词: アントシアニン; 配糖化酵素; アシル化酵素; 液胞; Zwitter donor; p-hydroxybenzyl-glucose; デルフィニウム; Delphinium grandiflorum; シロイヌナズナ; アシルグルコース; glycoside hydrolase family 1; シネラリア; アガパンサス; アシル-グルコース

Acyl-glucose dependent glucosyltransfrase (AAGT) gene was found in a monocot plant, Agapanthus, and Arabidopsis AAGT acted to acyl-modified anthocyanin with glucosyl residues in red turning leaves, indicating that AAGT reaction is common in the plant kingdom. In delphinium sepals, p-hydroxybenzyl-glucose (pHBG) acted as a glucosyl-donor at modification at the 7 position of anthocyanidin. The anthocyanin, violdelphin, consisted of three glycosyl and two p-hydroxybenzyl (pHBA)-residues, conjugated with alternately tandem and linear conjugation of glycosyl and pHBA-residues. In the reaction to form violdelphin, it was revealed that pHBG played a bi-functional role to be acyl donors and glucosyl donors for acyl-glucose dependent acyl-transferase and AAGT as Zwitter donor. The gene for UDP-glucose dependent pHBA glucosyltransferase was identified and the loss of this activity resulted not to modify at the 7 position of anthocyanidin.

在单子叶植物，agapanthus和拟南芥AAGT中发现了酰基葡萄糖依赖性葡萄糖基转磷酸酶（AAGT）基因，该基因作用于酰基修饰的花青素，在红旋转叶片中，葡萄糖基残基，表明AAGT在植物王国中很常见。在Delphinium萼片中，p-羟基苯甲酰葡萄糖（PHBG）在花青素的7位置在修饰时作为葡萄糖基含量。花色苷Violdelphin由三个糖基和两个P-羟基苯基（PHBA） - 分子组成，与糖基和PHBA疗养者的交替串联和线性共轭结合。在形成violdelphin的反应中，据揭示了PHBG在酰基葡萄糖依赖性酰基转移酶和AAGT作为Zwitter供体中发挥了双功能为酰基供体和葡萄糖基供体。鉴定了UDP-葡萄糖依赖性PHBA葡萄糖基转移酶的基因，并且该活性的丧失导致在花青素的7位置不改变。

项目成果

Rice Os9BGlu31 Is a Transglucosidase with the Capacity to Equilibrate Phenylpropanoid, Flavonoid, and Phytohormone Glycoconjugates

• DOI: 10.1074/jbc.m112.423533
• 发表时间: 2013-04-05
• 期刊: JOURNAL OF BIOLOGICAL CHEMISTRY
• 影响因子: 4.8
• 作者: Luang, Sukanya;Cho, Jung-Il;Cairns, James R. Ketudat
• 通讯作者: Cairns, James R. Ketudat

Comparison of signal enhancement techniques using DNA microarrays for screening GM crops.

使用 DNA 微阵列筛选转基因作物的信号增强技术的比较。

• 发表时间: 2013
• 期刊: Jpn. J. Food Chem. Safety
• 作者: Ito;A.
• 通讯作者: A.

Formation of volatile halogenated compounds in fresh-cut cabbage treated with sodium hypochlorite.

用次氯酸钠处理的鲜切卷心菜中挥发性卤代化合物的形成。

• 发表时间: 2012
• 期刊: Jpn. J. Food Chem. Safety
• 作者: Kubota;H.
• 通讯作者: H.

赤系デルフィニウムのアントシアニンの構造と F3’5’H 遺伝子の解析

红飞燕草花色苷结构及F35H基因分析

• 发表时间: 2013
• 作者: 宮川菜月;西崎雄三;宮原 平;廣瀬由起夫;岡本充智;小関良宏;佐々木伸大
• 通讯作者: 佐々木伸大

Determination of sodium stearoyl lactylates in foods using HPLC after derivatization with 2-nitrophenyl hydrazine.

采用 2-硝基苯肼衍生化后的 HPLC 法测定食品中的硬脂酰乳酸钠。

• 发表时间: 2013
• 期刊: Jpn. J. Food Chem. Safety
• 作者: Mikawa;T.
• 通讯作者: T.