Transcription regulatory factors in carrot suspension cultured cells regulated by auxin.

胡萝卜悬浮培养细胞中转录调控因子受生长素调控。

• 批准号: 06640835
• 负责人: OZEKI Yoshihiro
• 金额: $ 1.34万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06640835/
• 关键词: anthocyanin; carrot (Daucus carota); myb; plant cell culture; transcription regulatory factor; auxin; anthocyanin; carrot (Daucus carota); myb; plant cell culture; transcription regulatory factor; auxin

In order to elucidate the regulatory mechanisms of plant differentiation by plant growth regulators, transcription regulatory factors were investigated in carrot suspension cultured cells. In this research, myb related genes, one of transcription regulatory factors known as proto-oncogene in animal cells, were isolated from subtracted cDNA libraries of carrot suspension cultured cells induced for anthocyanin synthesis. Five clones of myb related genes (DCmyb8, DCmyb10, DCmyb12, DCmyb14 and DCmyb35) were isolated and their nucleotide sequences were determined. DCmyb10 and DCmyb12 were highly similar (98% similarity in nucleotide sequences), but the others had less identity than 55% in nucleotide sequences or 70% in translated amino acid sequences. Using specific nucleotide sequences of each myb related genes as probes, expression patterns of these genes were analyzed in carrot cultured cells under dedifferentiated growth, under differentiated states inducing for anthocyanin synthesis or embryogenesis, and in an anthocyanin-synthesizing mutant cell line or an embryogenic cell line of carrot callus cultures. Both DCmyb8 and DCmyb10 (12) expressed in anthocyanin-synthesizing cells under differentiated states. The expression of DCmyb10 was observed in a mutant cell line synthesizing anthocyanin, but that of DCmyb8 was not in it. DCmyb14 and DCmyb35 expressed in the cells under embryogenesis, especially DCmyb35 did in the cells of globular stage. The results obtained here suggested that myb genes isolated here expressed in cells under different differentiation states in different manners, and that the individual genes might regulate the expression of genes involved in different differentiation process.

为了阐明植物生长调节剂植物分化的调节机制，研究了胡萝卜悬浮培养的细胞中的转录调节因子。在这项研究中，与MYB相关的基因是动物细胞中称为原始癌基因的转录调节因子之一，是从诱导的花青素合成的胡萝卜悬浮培养细胞的减去cDNA文库中分离出来的。分离了MYB相关基因的五个克隆（DCMYB8，DCMYB10，DCMYB12，DCMYB14和DCMYB35），并确定其核苷酸序列。 DCMYB10和DCMYB12高度相似（核苷酸序列中的相似性为98％），但在核苷酸序列中的身份较小，而在翻译的氨基酸序列中的身份较小。使用每个MYB相关基因的特定核苷酸序列作为探针，在去分化的生长下，在分化的状态下，在诱导花道蛋白合成或胚胎发生的分化状态下以及在牛qus蛋白 - 蛋白酶合成的突变细胞系或胚胎细胞培养物中诱导花道蛋白的合成或胚胎的表达模式。 DCMYB8和DCMYB10（12）均在分化态下的花青素合成细胞中表达。 DCMYB10的表达在突变细胞系合成花青素中观察到，但DCMYB8的表达不在其中。 DCMYB14和DCMYB35在胚胎发生下在细胞中表达，尤其是DCMYB35在球体阶段的细胞中所做的。这里获得的结果表明，此处分离的MYB基因以不同的方式在不同分化状态下在细胞中表达的MYB基因，并且单个基因可能调节与不同分化过程有关的基因的表达。