Transcription regulatory factors in carrot suspension cultured cells regulated by auxin.

胡萝卜悬浮培养细胞中转录调控因子受生长素调控。

基本信息

批准号：
06640835
负责人：
OZEKI Yoshihiro
金额：
$ 1.34万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06640835/
关键词：
anthocyanin carrot (Daucus carota)myb plant cell culture transcription regulatory factor auxin

项目摘要

In order to elucidate the regulatory mechanisms of plant differentiation by plant growth regulators, transcription regulatory factors were investigated in carrot suspension cultured cells. In this research, myb related genes, one of transcription regulatory factors known as proto-oncogene in animal cells, were isolated from subtracted cDNA libraries of carrot suspension cultured cells induced for anthocyanin synthesis. Five clones of myb related genes (DCmyb8, DCmyb10, DCmyb12, DCmyb14 and DCmyb35) were isolated and their nucleotide sequences were determined. DCmyb10 and DCmyb12 were highly similar (98% similarity in nucleotide sequences), but the others had less identity than 55% in nucleotide sequences or 70% in translated amino acid sequences. Using specific nucleotide sequences of each myb related genes as probes, expression patterns of these genes were analyzed in carrot cultured cells under dedifferentiated growth, under differentiated states inducing for anthocyanin synthesis or embryogenesis, and in an anthocyanin-synthesizing mutant cell line or an embryogenic cell line of carrot callus cultures. Both DCmyb8 and DCmyb10 (12) expressed in anthocyanin-synthesizing cells under differentiated states. The expression of DCmyb10 was observed in a mutant cell line synthesizing anthocyanin, but that of DCmyb8 was not in it. DCmyb14 and DCmyb35 expressed in the cells under embryogenesis, especially DCmyb35 did in the cells of globular stage. The results obtained here suggested that myb genes isolated here expressed in cells under different differentiation states in different manners, and that the individual genes might regulate the expression of genes involved in different differentiation process.

为了阐明植物生长调节剂对植物分化的调节机制，对胡萝卜悬浮培养细胞中的转录调节因子进行了研究。本研究从诱导花青素合成的胡萝卜悬浮培养细胞的消减cDNA文库中分离出myb相关基因，它是动物细胞中被称为原癌基因的转录调节因子之一。分离了myb相关基因的五个克隆（DCmyb8、DCmyb10、DCmyb12、DCmyb14和DCmyb35）并测定了它们的核苷酸序列。 DCmyb10和DCmyb12高度相似（核苷酸序列相似性为98%），但其他的核苷酸序列同一性低于55%，翻译氨基酸序列同一性低于70%。使用每个myb相关基因的特定核苷酸序列作为探针，分析了这些基因在去分化生长下、诱导花青素合成或胚胎发生的分化状态下以及在花青素合成突变细胞系或胚胎发生细胞中的胡萝卜培养细胞中的表达模式。胡萝卜愈伤组织培养物系列。 DCmyb8 和 DCmyb10 (12) 在分化状态下的花青素合成细胞中表达。在合成花青素的突变细胞系中观察到DCmyb10的表达，但其中没有DCmyb8的表达。 DCmyb14和DCmyb35在胚胎发生期细胞中表达，尤其是DCmyb35在球状期细胞中表达。本文获得的结果表明，本文分离的myb基因在不同分化状态的细胞中以不同方式表达，并且各个基因可能调节参与不同分化过程的基因的表达。