Production of cytoplasmic male sterile and its maintainer by using asymmetrical protoplast fusion and incorporation of isolated nuclei.

通过使用不对称原生质体融合和分离细胞核的掺入来产生细胞质雄性不育及其维持系。

基本信息

批准号：
63560001
负责人：
MII Masahiro
金额：
$ 1.09万
依托单位：
Chiba University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1988
资助国家：
日本
起止时间：
1988 至 1989
项目状态：
已结题

项目摘要

Introduction of cytoplasmic male sterility into normal fertile plants and vice versa were tried by using asymmetrical protoplast fusion technique and incorporation of isolated nuclei in carrot.Both donor and recipient cell lines of cytoplasm were fused by PEG treatment after irradiation with 60 kR x-ray and treatment with 10 mM iodoactamide, respectively. Irrespective of the cell materials used, many plantlets were regenerated from the fusion products via somatic embryogenesis. However, no plants with altered male sterility were obtained. In the control cultures treated with only x-ray or with IOA, some escaped colonies were obtained presumably due to the complementation. Therefore, stronger treatments were applied to suppress the escape before fusion treatments. These treatments reduced the number of survived colonies which eventually developed into embryos. Further experiments are now in progress.Isolation of nuclei from the embryogenic cell cultures was extremely difficult, whereas that from vacuolated non-embryogenic cells was relatively easy. However, the use of nuclei isolated from non-embryogenic cells was not suitable for obtaining plant regeneration after nuclei incorporation. Further experiment is required to get nuclei from embryogenic cells.

通过使用不对称的原生质体融合技术并在胡萝卜中使用不对称的原生质体融合技术并掺入分离的核。在胡萝卜中尝试将细胞质雄性不育引入正常肥沃的植物中。在辐射后通过PEG处理进行60 kr X射线和10 mmMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMM。无论使用的细胞材料如何，许多植物会通过体细胞胚发生从融合产物中再生。但是，没有获得雄性无菌性改变的植物。在仅用X射线或IOA处理的对照培养物中，大概是由于补充而获得的一些逃脱菌落。因此，在融合处理之前，采用更强大的治疗方法来抑制逃生。这些治疗方法减少了最终发展为胚胎的幸存菌落的数量。现在正在进行进一步的实验。从胚胎细胞培养物中的核分离非常困难，而从空泡的非胚胎细胞中进行的核化是相对容易的。然而，从非胚胎细胞中分离出的核不适合在核掺入后获得植物再生。需要进一步的实验才能从胚胎细胞中获取核。